用于分离和捕获源自脂肪肉瘤细胞条件培养基的细胞外囊泡的多层微纳流体装置

Sundaram Prashanth Mohana, Casadei Lucia, Lopez Gonzalo, Braggio Danielle, Balakirsky Gita, Pollock Raphael, Prakash Shaurya

Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210 USA.

Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210 USA.

J Microelectromech Syst. 2020 Oct;29(5):776-782. doi: 10.1109/jmems.2020.3006786. Epub 2020 Jul 14.

We report on isolation, capture, and subsequent elution for analysis of extracellular vesicles derived from human liposarcoma cell conditioned media, using a multi-layer micro-nanofluidic device operated with tangential flow separation. Our device integrates size-based separation followed by immunoaffinity-based capture of extracellular vesicles in the same device. For liposarcomas, this is the first report on isolating, capturing, and then eluting the extracellular vesicles using a micro-nanofluidic device. The results show a significantly higher yield of the eluted extracellular vesicles (~~84%) compared to the current methods of ultracentrifugation (~~6%) and ExoQuick-based separations (~16%).

我们报告了使用切向流分离操作的多层微纳流体装置，对源自人脂肪肉瘤细胞条件培养基的细胞外囊泡进行分离、捕获及后续洗脱以进行分析的过程。我们的装置在同一设备中集成了基于尺寸的分离，随后是基于免疫亲和的细胞外囊泡捕获。对于脂肪肉瘤，这是首次使用微纳流体装置分离、捕获并洗脱细胞外囊泡的报告。结果显示，与目前的超速离心法（约6%）和基于ExoQuick的分离法（约16%）相比，洗脱的细胞外囊泡产量显著更高（约84%）。