Zhang Yiru, Shen Jianlei, Hu Rong, Shi Xiujuan, Hu Xianglong, He Benzhao, Qin Anjun, Tang Ben Zhong
State Key Laboratory of Luminescent Materials and Devices, Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates, Center for Aggregation-Induced Emission, South China University of Technology Guangzhou 510640 China
Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Centre for Tissue Restoration and Reconstruction, Institute for Advanced Study, and Department of Chemical and Biological Engineering, The Hong Kong University of Science & Technology Clear Water Bay Kowloon Hong Kong China
Chem Sci. 2020 Mar 18;11(15):3931-3935. doi: 10.1039/d0sc00062k.
Surface immobilization provides a useful platform for biosensing, drug screening, tissue engineering and other chemical and biological applications. However, some of the used reactions are inefficient and/or complicated, limiting their applications in immobilization. Herein, we use a spontaneous and catalyst-free amino-yne click bioconjugation to generate activated ethynyl group functionalized surfaces for fast immobilization of native proteins and cells. Biomolecules, such as bovine serum albumin (BSA), human IgG and a peptide of C(RGDfK), could be covalently immobilized on the surfaces in as short as 30 min. Notably, the bioactivity of the anchored biomolecules remains intact, which is verified by efficiently capturing target antibodies and cells from the bulk solutions. This strategy represents an alternative for highly efficient surface biofunctionalization.
表面固定化为生物传感、药物筛选、组织工程及其他化学和生物学应用提供了一个有用的平台。然而，一些已使用的反应效率低下和/或复杂，限制了它们在固定化方面的应用。在此，我们使用一种自发且无催化剂的氨基-炔点击生物共轭反应来生成活化乙炔基功能化表面，用于快速固定天然蛋白质和细胞。生物分子，如牛血清白蛋白（BSA）、人免疫球蛋白G（IgG）和C(RGDfK)肽，可在短短30分钟内共价固定在表面。值得注意的是，锚定生物分子的生物活性保持完整，这通过从大量溶液中有效捕获靶抗体和细胞得到验证。该策略代表了一种高效表面生物功能化的替代方法。
