Li Yan, Liu Junye, Liu Xinping, Xing Kefei, Wang Yun, Li Fuyang, Yao Libo
Department of Biochemistry and Molecular Biology and The State Key Laboratory of Cancer Biology, The Fourth Military Medical University, Xi'an 710032, China.
Appl Biochem Biotechnol. 2006 Dec;135(3):181-92. doi: 10.1385/abab:135:3:181.
Resveratrol (trans-3,4N,-5-trihydroxystilbene), a phytoalexin present in grapes and red wine, is emerging as a natural compound with potential anticancer properties. Here we show that resveratrol affects the growth of human breast cancer cell lines MCF7, MDA-MB-231, SK-BR-3, and Bcap-37 in a dose-dependent manner and that MCF7 is the most sensitive among the four cell lines. MCF7 cells treated with resveratrol showed typical characteristics of apoptosis including the poly (ADP-ribose) polymerase cleavage, TdT-mediated dUTP nick end labeling-positive staining, and morphologic changes. Phosphorylation of the oncogene product Akt was significantly reduced followed by decreased phosphorylation and increased processing of pro-caspase-9 on resveratrol treatment. These results indicate that resveratrol seems to exert its growth-inhibitory/apoptotic effect on the breast cancer cell line MCF7 via the Akt-caspase-9 pathway.
白藜芦醇（反式-3,4′,5-三羟基芪）是一种存在于葡萄和红酒中的植物抗毒素，正逐渐成为一种具有潜在抗癌特性的天然化合物。在此我们表明，白藜芦醇以剂量依赖的方式影响人乳腺癌细胞系MCF7、MDA-MB-231、SK-BR-3和Bcap-37的生长，且MCF7是这四种细胞系中最敏感的。用白藜芦醇处理的MCF7细胞表现出典型的凋亡特征，包括多聚（ADP-核糖）聚合酶裂解、TdT介导的dUTP缺口末端标记阳性染色以及形态学变化。癌基因产物Akt的磷酸化显著降低，随后在白藜芦醇处理后，原半胱天冬酶-9的磷酸化减少且加工增加。这些结果表明，白藜芦醇似乎通过Akt-半胱天冬酶-9途径对乳腺癌细胞系MCF7发挥其生长抑制/凋亡作用。
