Vascellari Marta, Nonno Romolo, Mutinelli Franco, Bigolaro Michela, Di Bari Michele Angelo, Melchiotti Erica, Marcon Stefano, D'Agostino Claudia, Vaccari Gabriele, Conte Michela, De Grossi Luigi, Rosone Francesca, Giordani Francesco, Agrimi Umberto
Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Università 10, 35020 Legnaro (PD), Italy.
J Virol. 2007 May;81(9):4872-6. doi: 10.1128/JVI.02148-06. Epub 2007 Feb 14.
The salivary glands of scrapie-affected sheep and healthy controls were investigated for the presence of the pathological prion protein (PrP(Sc)). PrP(Sc) was detected in major (parotid and mandibular) and minor (buccal, labial, and palatine) salivary glands of naturally and experimentally infected sheep. Using Western blotting, the PrP(Sc) concentration in glands was estimated to be 0.02 to 0.005% of that in brain. Immunohistochemistry revealed intracellular depositions of PrP(Sc) in ductal and acinar epithelia and occasional labeling in the lumina of salivary ducts. The presence of PrP(Sc) in salivary glands highlights the possible role of saliva in the horizontal transmission of scrapie.
对患瘙痒病绵羊和健康对照的唾液腺进行了研究，以检测病理性朊病毒蛋白（PrP(Sc)）的存在情况。在自然感染和实验感染绵羊的主要唾液腺（腮腺和下颌下腺）以及次要唾液腺（颊腺、唇腺和腭腺）中均检测到了PrP(Sc)。通过蛋白质印迹法，估计腺体中PrP(Sc)的浓度为脑中浓度的0.02%至0.005%。免疫组织化学显示，PrP(Sc)在导管和腺泡上皮细胞内沉积，并且在唾液导管管腔中偶尔有标记。唾液腺中存在PrP(Sc)突出了唾液在瘙痒病水平传播中可能发挥的作用。
