Carlsson L, Overmo C, Holmberg D
Unit for Applied Cell and Molecular Biology, University of Umeå, Sweden.
Eur J Immunol. 1992 Jan;22(1):71-8. doi: 10.1002/eji.1830220112.
Nucleotide sequence analysis of a large number of rearranged immunoglobulin heavy chain V region genes allowed the identification of six new members of the VH7183 gene family. These six new genes plus the eight previously defined genes agrees with the previously estimated complexity of this gene family. Twelve of these genes were represented among the isolated clones. A comparison of the clones, derived from 1-day- and 14-week-old BALB/c mice, suggested a biased and developmentally controlled VH7183 gene utilization. Furthermore, a developmentally controlled, non-random distribution of the functional vs. non-functional VHDJH rearrangements was observed among clones utilizing genes of this family, suggesting unsuspected regulatory aspects of Ig rearrangements in the process of B cell differentiation. Finally, a limited junctional diversity was revealed among the neonatal clones as the result of a low frequency of N-sequence addition. A similar discrepancy was also observed between neonatal and adult VHJ558 clones. In conclusion, these data suggest a programmed generation of B cell diversity similar to what has been observed for the establishment of gamma/delta T cell repertoires.
对大量重排的免疫球蛋白重链V区基因进行核苷酸序列分析，使得VH7183基因家族的六个新成员得以鉴定。这六个新基因加上先前定义的八个基因，与该基因家族先前估计的复杂性相符。其中十二个基因在分离的克隆中得以体现。对来自1日龄和14周龄BALB/c小鼠的克隆进行比较，提示VH7183基因利用存在偏向性且受发育控制。此外，在利用该家族基因的克隆中，观察到功能性与非功能性VHDJH重排在发育上受控制的非随机分布，提示B细胞分化过程中Ig重排存在未被怀疑的调控方面。最后，由于N序列添加频率低，在新生克隆中揭示了有限的连接多样性。在新生和成年VHJ558克隆之间也观察到类似差异。总之，这些数据提示B细胞多样性的程序性产生，类似于在γ/δT细胞库建立过程中所观察到的情况。
