Zuniga Armando, Wang Zili, Liniger Matthias, Hangartner Lars, Caballero Michael, Pavlovic Jovan, Wild Peter, Viret Jean Francois, Glueck Reinhard, Billeter Martin A, Naim Hussein Y
Institute of Molecular Biology, University of Zürich, Zürich, Switzerland.
Vaccine. 2007 Apr 20;25(16):2974-83. doi: 10.1016/j.vaccine.2007.01.064. Epub 2007 Feb 2.
Live attenuated measles virus (MV) vaccines have an impressive record of safety, efficacy and ability to induce life-long immunity against measles infection. Using reverse genetics technology, such negative-strand RNA viruses can now be rescued from cloned DNA. This technology allows the insertion of exogenous genes encoding foreign antigens into the MV genome in such a way that they can be expressed by the MV vaccine strain, without affecting virus structure, propagation and cell targeting. Recombinant viruses rescued from cloned cDNA induce immune responses against both measles virus and the cloned antigens. The tolerability of MV to gene(s) insertion makes it an attractive flexible vector system, especially if broad immune responses are required. The fact that measles replication strictly occurs in the cytoplasm of infected cells without DNA intermediate has important biosafety implications and adds to the attractiveness of MV as a vector. In this article we report the characteristics of reporter gene expression (GFP, LacZ and CAT) and the biochemical, biophysical and immunological properties of recombinant MV expressing heterologous antigens of simian immunogeficiency virus (SIV).
减毒活麻疹病毒（MV）疫苗在安全性、有效性以及诱导针对麻疹感染的终身免疫力方面有着令人瞩目的记录。利用反向遗传学技术，现在可以从克隆的DNA中拯救出这种负链RNA病毒。这项技术允许将编码外源抗原的基因插入MV基因组，使其能够由MV疫苗株表达，而不影响病毒结构、繁殖和细胞靶向性。从克隆的cDNA中拯救出的重组病毒可诱导针对麻疹病毒和克隆抗原的免疫反应。MV对基因插入的耐受性使其成为一个有吸引力的灵活载体系统，特别是在需要广泛免疫反应的情况下。麻疹病毒在受感染细胞的细胞质中严格复制且无DNA中间体这一事实具有重要的生物安全意义，并增加了MV作为载体的吸引力。在本文中，我们报告了报告基因（绿色荧光蛋白、β-半乳糖苷酶和氯霉素乙酰转移酶）表达的特征以及表达猿猴免疫缺陷病毒（SIV）异源抗原的重组MV的生化、生物物理和免疫学特性。
