黄褥花树叶水提取物的抗伤害感受作用：阿片受体的作用
The antinociceptive action of aqueous extract from Muntingia calabura leaves: the role of opioid receptors.
作者信息
Zakaria Zainul Amiruddin, Mustapha Safarul, Sulaiman Mohd Roslan, Mat Jais Abdul Manan, Somchit Muhammad Nazrul, Abdullah Fatimah Corazon
机构信息
Faculty of Biotechnology and Life Sciences, Universiti Industri Selangor, Selangor, Malaysia.
出版信息
Med Princ Pract. 2007;16(2):130-6. doi: 10.1159/000098366.
OBJECTIVE
The present study was carried out to investigate the antinociceptive activity of the aqueous extract of Muntingia calabura (MCAE) leaves and to determine the effect of temperature and the involvement of the opioid receptor on the said activity using the abdominal constriction test (ACT) and hot-plate test (HPT) in mice.
MATERIALS AND METHODS
The extract was prepared by soaking the dried powdered leaves of M. calabura in distilled water (dH(2)O) overnight, and the supernatant obtained was considered as a stock solution with 100% concentration. The stock solution was diluted to 1, 5, 10, 50 and 100% and used to determine the antinociceptive activity of MCAE. A further experiment was done with 50% concentration to determine the effect of temperature and naloxone involvement of the opioid receptor system in MCAE antinociceptive activity.
RESULTS
At the various concentrations MCAE showed significant antinociceptive activity in both tests. However, the concentration-dependent activity was observed only in the ACT but not in the HPT. The 50% concentration of MCAEs were also stable against the effect of various temperatures as indicated by the presence of activity in both tests. The temperatures (40, 60 and 100 degrees C) also showed an enhanced extract activity only in the HPT. Pre-treatment with naloxone (2 and 10 mg/kg) blocked the extract activity in both tests, indicating the involvement of the opioid receptor system in MCAE antinociceptive activity.
CONCLUSION
Our data indicate that M. calabura leaves possess antinociceptive activity against chemically and thermally induced noxious stimuli. The bioactive compound(s) responsible for its antinociceptive activity is/are heat-stable and work partly via the opioid receptor system.
目的
本研究旨在通过小鼠腹部收缩试验（ACT）和热板试验（HPT），研究巴拉圭樱桃（Muntingia calabura）叶水提取物（MCAE）的抗伤害感受活性，并确定温度的影响以及阿片受体在上述活性中的作用。
材料与方法
将巴拉圭樱桃干燥的粉末状叶子浸泡在蒸馏水中过夜制备提取物，所得上清液被视为浓度为100%的储备溶液。将储备溶液稀释至1%、5%、10%、50%和100%，用于测定MCAE的抗伤害感受活性。用50%浓度进行进一步实验，以确定温度的影响以及阿片受体系统中纳洛酮对MCAE抗伤害感受活性的影响。
结果
在两种试验中，不同浓度的MCAE均显示出显著的抗伤害感受活性。然而，仅在ACT中观察到浓度依赖性活性，而在HPT中未观察到。50%浓度的MCAE在两种试验中均有活性，表明其对不同温度的影响具有稳定性。温度（40、60和100℃）仅在HPT中增强了提取物的活性。用纳洛酮（2和10mg/kg）预处理可阻断两种试验中的提取物活性，表明阿片受体系统参与了MCAE的抗伤害感受活性。
结论
我们的数据表明，巴拉圭樱桃叶对化学和热诱导的有害刺激具有抗伤害感受活性。其抗伤害感受活性的生物活性化合物是热稳定的，并且部分通过阿片受体系统发挥作用。
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