从广玉兰中筛选和分离一种杀线虫倍半萜

Screening and isolation of a nematicidal sesquiterpene from Magnolia grandiflora L.

作者信息

Hong Linjun, Li Guohong, Zhou Wei, Wang Xinbiao, Zhang Keqin

机构信息

Laboratory for Conservation and Utilization of Bioresources, Yunnan University, Kunming 650091, PR China.

出版信息

Pest Manag Sci. 2007 Mar;63(3):301-5. doi: 10.1002/ps.1337.

DOI:10.1002/ps.1337

PMID:17304632

Abstract

The ethanolic extracts from 30 plant species were tested for their nematicidal activity against nematodes Bursaphelenchus xylophilus (Steiner & Buhrer) Nickle and Panagrellus redivivus (L.) Goodey. The leaf extract of Magnolia grandiflora L. exhibited the strongest nematicidal activity against both nematodes, causing 73 and 100% mortality respectively within 48 h at 5 mg mL(-1). A new nematicidal sesquiterpene was obtained from the leaves of M. grandiflora. The compound was determined to be 4,5-epoxy-1(10)E,11(13)-germacradien-12,6-olide, based on spectroscopic methods including 2D NMR techniques. The median lethal concentrations (LC50) of the compound against B. xylophilus and P. redivivus were 71 and 46 mg L(-1) respectively at 48 h. This is the first report of Magnoliaceae species with nematicidal activity.

摘要

测试了30种植物的乙醇提取物对松材线虫（Steiner & Buhrer）Nickle和小杆线虫（L.）Goodey的杀线虫活性。广玉兰叶提取物对两种线虫均表现出最强的杀线虫活性，在5 mg mL(-1)浓度下，48小时内死亡率分别为73%和100%。从广玉兰叶中获得了一种新的杀线虫倍半萜。基于包括二维核磁共振技术在内的光谱方法，该化合物被确定为4,5-环氧-1(10)E,11(13)-大根香叶二烯-12,6-内酯。该化合物在48小时时对松材线虫和小杆线虫的半数致死浓度（LC50）分别为71和46 mg L(-1)。这是木兰科植物具有杀线虫活性的首次报道。