一种来自石榴皮的杀线虫单宁及其对松材线虫（Bursaphelenchus xylophilus）的生理作用。

A nematicidal tannin from Punica granatum L. rind and its physiological effect on pine wood nematode (Bursaphelenchus xylophilus).

作者信息

Guo Qunqun, Du Guicai, Qi Hongtao, Zhang Yanan, Yue Tongqing, Wang Jingchao, Li Ronggui

机构信息

College of Life Sciences, Qingdao University, Qingdao 266071, PR China.

出版信息

Pestic Biochem Physiol. 2017 Jan;135:64-68. doi: 10.1016/j.pestbp.2016.06.003. Epub 2016 Jun 8.

DOI:10.1016/j.pestbp.2016.06.003

PMID:28043333

Abstract

The ethanol extract of Punica granatum L. rind was tested to show significant nematicidal activity against pine wood nematode. Three nematicidal compounds were obtained from the ethanol extract by bioassay-guided fractionation and identified as punicalagin 1, punicalin 2, and corilagin 3 by mass and nuclear magnetic resonance spectral data analysis. Punicalagin 1 was most active against PWN among the purified compounds with the LC value of 307.08μM in 72h. According to the enzyme assays in vitro, punicalagin 1 could inhibit the activity of acetylcholinesterase, amylase and cellulase from PWN with IC value of 0.60mM, 0.96mM and 1.24mM, respectively. The morphological structures of PWNs treated by punicalagin 1 were greatly changed. These physiological effects of punicalagin 1 on PWN may helpful to elucidate its nematicidal mechanism.

摘要

石榴皮乙醇提取物经测试显示对松材线虫具有显著的杀线虫活性。通过生物测定导向分级分离从乙醇提取物中获得了三种杀线虫化合物，并通过质谱和核磁共振光谱数据分析鉴定为石榴皮苷1、石榴皮素2和鞣花酸3。在纯化的化合物中，石榴皮苷1对松材线虫活性最高，72小时内的LC值为307.08μM。根据体外酶活性测定，石榴皮苷1可抑制松材线虫乙酰胆碱酯酶、淀粉酶和纤维素酶的活性，IC值分别为0.60mM、0.96mM和1.24mM。经石榴皮苷1处理的松材线虫的形态结构发生了很大变化。石榴皮苷1对松材线虫的这些生理作用可能有助于阐明其杀线虫机制。