Rath N C, Kannan L, Pillai P B, Huff W E, Huff G R, Horst R L, Emmert J L
Agricultural Research Service, USDA, Poultry Science Center, University of Arkansas, Fayetteville, AR 72701, United States.
Res Vet Sci. 2007 Oct;83(2):244-50. doi: 10.1016/j.rvsc.2006.12.008. Epub 2007 Feb 20.
Two trials were conducted to determine if thiram-induced tibial dyschondroplasia (TD) in chickens was linked to a vitamin D deficiency and calcium homeostasis dysregulation, and whether feeding vitamin D fortified diets may prevent it. Day-old chickens were given grower diets containing different vitamin D products throughout the experiment until necropsy on day 16. Half of the birds in each feed group received thiram at levels of 100 ppm (trial 1) or 50 ppm (trial 2) between days 7-9 to induce TD. The birds were weighed, bled, and euthanized to determine TD incidences and severity by examining the growth plates. Tibial bones were used to measure biomechanical strength and ash content. Blood concentrations of 25-hydroxyvitamin D, Ca, P, alkaline phosphatase, and creatine kinase were measured in serum that showed no differences between different groups. Thiram reduced body weight and induced TD regardless of any vitamin D treatment to the same extent as untreated birds.
进行了两项试验，以确定福美双诱导的鸡胫骨软骨发育不良（TD）是否与维生素D缺乏和钙稳态失调有关，以及喂食富含维生素D的日粮是否可以预防该病。在整个实验过程中，给一日龄雏鸡饲喂含有不同维生素D产品的生长日粮，直至第16天进行尸检。每个饲料组中一半的鸡在第7至9天接受100 ppm（试验1）或50 ppm（试验2）水平的福美双以诱导TD。对鸡进行称重、采血并实施安乐死，通过检查生长板来确定TD的发生率和严重程度。使用胫骨测量生物力学强度和灰分含量。在血清中测量25-羟基维生素D、钙、磷、碱性磷酸酶和肌酸激酶的血浓度，结果显示不同组之间无差异。无论进行何种维生素D处理，福美双都会使体重减轻并诱导TD，其程度与未处理的鸡相同。
