Bonini S, Bonini S, Schiavone M, Centofanti M, Allansmith M R, Bucci M G
Department of Ophthalmology, University of Rome Tor Vergata, Italy.
J Allergy Clin Immunol. 1992 Jan;89(1 Pt 1):103-7. doi: 10.1016/s0091-6749(05)80046-6.
We compared the conjunctival responsiveness to histamine diphosphate in patients with vernal conjunctivitis and in healthy control subjects. Fourteen asymptomatic patients with vernal conjunctivitis and 10 healthy volunteers were challenged in one eye with 10 microliters of increasing doses (0.01, 0.05, 0.5, and 1 mg/ml in phosphate-buffered saline) of histamine diphosphate. The contralateral eye was challenged with the diluent only. Photographs of each eye were taken for evaluation of conjunctival redness by two masked investigators. Both patients with vernal conjunctivitis and control subjects reacted to histamine with a dose-dependent conjunctival redness 2 to 5 minutes after ocular challenge. Conjunctival redness was more intense in patients with vernal conjunctivitis than in control subjects after ocular challenge with 0.01 and 0.05 mg/ml of histamine diphosphate solution (p less than 0.05). Moreover, the threshold concentration of histamine diphosphate, extrapolated from each individual dose-response curve, was significantly (p less than 0.02) lower in patients with vernal conjunctivitis than in control subjects. Our findings suggest that patients with vernal conjunctivitis demonstrate conjunctival hyperresponsiveness to a nonspecific challenging agent. Nonspecific conjunctival hyperreactivity, a novel concept in allergic eye disease, may be relevant for a better understanding of the pathogenesis and clinical variability of vernal conjunctivitis.
我们比较了春季结膜炎患者和健康对照者结膜对磷酸组胺的反应性。14例无症状春季结膜炎患者和10名健康志愿者的一只眼睛接受了10微升递增剂量（在磷酸盐缓冲盐水中分别为0.01、0.05、0.5和1毫克/毫升）的磷酸组胺刺激。对侧眼睛仅用稀释剂刺激。两名不知情的研究人员拍摄每只眼睛的照片以评估结膜充血情况。春季结膜炎患者和对照者在眼部刺激后2至5分钟均对组胺产生剂量依赖性结膜充血反应。在用0.01和0.05毫克/毫升磷酸组胺溶液进行眼部刺激后，春季结膜炎患者的结膜充血比对照者更强烈（p<0.05）。此外，从每条个体剂量反应曲线推断出的磷酸组胺阈值浓度，春季结膜炎患者显著低于对照者（p<0.02）。我们的研究结果表明，春季结膜炎患者对非特异性刺激剂表现出结膜高反应性。非特异性结膜高反应性是变应性眼病中的一个新概念，可能有助于更好地理解春季结膜炎的发病机制和临床变异性。
