Joo Chulmin, Kim Ki Hean, de Boer Johannes F
Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge 02139, USA.
Opt Lett. 2007 Mar 15;32(6):623-5. doi: 10.1364/ol.32.000623.
We describe simultaneous quantitative phase contrast and multiphoton fluorescence imaging by combined spectral-domain optical coherence phase and multiphoton microscopy. The instrument employs two light sources for efficient optical coherence microscopic and multiphoton imaging and can generate structural and functional images of transparent specimens in the epidirection. Phase contrast imaging exhibits spatial and temporal phase stability in the subnanometer range. We also demonstrate the visualization of actin filaments in a fixed cell specimen, which is confirmed by simultaneous multiphoton fluorescence imaging.
我们描述了通过组合光谱域光学相干相位和多光子显微镜实现的同步定量相衬和多光子荧光成像。该仪器采用两个光源进行高效的光学相干显微镜和多光子成像，并能在表皮方向生成透明标本的结构和功能图像。相衬成像在亚纳米范围内表现出空间和时间相位稳定性。我们还展示了固定细胞标本中肌动蛋白丝的可视化，这通过同步多光子荧光成像得到了证实。
