Vrielink H, Zaaijer H L, Cuypers H T, van der Poel C L, Woerdeman M, Lelie P N, Winkel C, Reesink H W
Red Cross Blood Bank Amsterdam, The Netherlands.
Vox Sang. 1997;72(3):144-7. doi: 10.1046/j.1423-0410.1997.7230144.x.
Evaluation of a qualitative HTLV-I/II DNA polymerase chain reaction (PCR) test for the detection of HTLV-I/II DNA (Roche Diagnostic Systems, Branchburg, N.J., USA) in various panels.
The panels consisted of fresh EDTA blood samples from blood donors who were anti-HTLV-I/II ELISA repeatably reactive: 53 were Western blot (WB) positive, 228 were WB indeterminate and 15 were WB negative. Elevent ELISA-negative blood donors were used as negative controls. Furthermore, specimens from 1 HTLV-II-infected intravenous drug user and from 1 HTLV-II-infected blood donor were included in the panel. Peripheral blood lymphocytes were prepared by red blood cell lysis with the Roche washing solution and stored at < -23 degrees C until processing. Amplification products were analyzed with the HTLV-I/II detection kit.
All 53 anti-HTLV-I/II ELISA- and WB-positive samples and both HTLV-II-positive samples tested positively by PCR. All 228 anti-HTLV-I/II ELISA-positive and WB-indeterminate, all 15 ELISA-positive and WB-negative and all II ELISA-negative control samples tested negative by PCR.
The Roche Amplicor HTLV-I/II test is a simple test, suitable for the confirmation of HTLV-I and-II infection in individuals with indeterminate or positive WB patterns.
评估一种定性的人嗜T淋巴细胞病毒I/II型（HTLV-I/II）DNA聚合酶链反应（PCR）检测方法，用于检测不同样本组中的HTLV-I/II DNA（罗氏诊断系统公司，美国新泽西州布兰奇堡）。
样本组包括来自抗HTLV-I/II酶联免疫吸附测定（ELISA）反复呈阳性反应的献血者的新鲜乙二胺四乙酸（EDTA）血液样本：53例免疫印迹法（WB）阳性，228例WB结果不确定，15例WB阴性。11例ELISA阴性的献血者用作阴性对照。此外，样本组还包括1例感染HTLV-II的静脉吸毒者和1例感染HTLV-II的献血者的样本。用罗氏洗涤液通过红细胞裂解制备外周血淋巴细胞，并在-23℃以下储存直至处理。用HTLV-I/II检测试剂盒分析扩增产物。
所有53例抗HTLV-I/II ELISA和WB阳性样本以及2例HTLV-II阳性样本经PCR检测均为阳性。所有228例抗HTLV-I/II ELISA阳性且WB结果不确定、所有15例ELISA阳性且WB阴性以及所有11例ELISA阴性对照样本经PCR检测均为阴性。
罗氏Amplicor HTLV-I/II检测是一种简单的检测方法，适用于确认WB结果不确定或阳性个体的HTLV-I和-II感染。
