Kozlovsky Avital, Artzi Zvi, Hirshberg Avraham, Israeli-Tobias Chen, Reich Liat
Department of Periodontology, The Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel.
J Clin Periodontol. 2007 Feb;34(2):164-71. doi: 10.1111/j.1600-051X.2006.01033.x.
To assess the effect of topically applied antimicrobial agents on palatal excisional wound in rats.
Excisional wounds, 5 mm in diameter, were made in the centre of the palate of 125 Wistar male rats. In four experimental groups, chlorhexidine digluconate (CHX) 0.12% solution, 1% CHX gel, phenolic compounds solution (Listerine), amine/stannous fluoride solution (Meridol) and saline solution as a control group were applied daily for 1 min. The wound area was measured photographically and the epithelialization rate was determined histologically at 3, 7, 14 and 21 days post-surgery.
The mean wound area and mean distance between the epithelial margins decreased significantly with time (p<0.001) in experimental and control groups, with the greatest wound area reduction and rate of epithelialization on day 14. A significantly superior rate of wound epithelialization (p=0.03) was presented following use of 1% CHX gel and Listerine and a comparatively inferior one when the Meridol solution was applied.
Each tested antimicrobial agent when applied on an excisional wound with epithelial and connective tissue deficiency did not have a negative effect on the rate of wound closure. The best results were achieved with 1%CHX gel and Listerine.
评估局部应用抗菌剂对大鼠腭部切除伤口的影响。
在125只雄性Wistar大鼠的腭部中央制作直径5毫米的切除伤口。在四个实验组中，每天应用0.12%葡萄糖酸氯己定（CHX）溶液、1%CHX凝胶、酚类化合物溶液（李施德林）、胺/氟化亚锡溶液（美利齿）1分钟，并用生理盐水作为对照组。术后3、7、14和21天通过摄影测量伤口面积，并通过组织学方法确定上皮化率。
实验组和对照组的平均伤口面积和上皮边缘之间的平均距离均随时间显著减小（p<0.001），在第14天时伤口面积减小最大且上皮化率最高。使用1%CHX凝胶和李施德林后伤口上皮化率显著更高（p=0.03），而应用美利齿溶液时上皮化率相对较低。
将每种测试的抗菌剂应用于存在上皮和结缔组织缺损的切除伤口时，对伤口闭合率均无负面影响。使用1%CHX凝胶和李施德林取得了最佳效果。
