通过稳定同位素标记对组蛋白翻译后修饰进行定量分析。
Quantitative profiling of histone post-translational modifications by stable isotope labeling.
作者信息
Knapp Amy R, Ren Chen, Su Xiaodan, Lucas David M, Byrd John C, Freitas Michael A, Parthun Mark R
机构信息
Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus, OH 43210, USA.
出版信息
Methods. 2007 Mar;41(3):312-9. doi: 10.1016/j.ymeth.2006.08.017.
Abstract
Methods for accurately quantitating changes in histone post-translational modifications are necessary for developing an understanding of how their dynamic nature influences nuclear events involving access to genomic DNA. This article describes methods for the use of in vivo stable isotope label incorporation for quantitating the levels of modification at specific residues in histone proteins. These methods are applicable to a wide variety of model systems and examples of their use in both mammalian cells and Saccharomyces cerevisiae are presented.
摘要
准确量化组蛋白翻译后修饰变化的方法对于理解其动态性质如何影响涉及基因组DNA获取的核事件是必要的。本文描述了使用体内稳定同位素标记掺入来量化组蛋白特定残基修饰水平的方法。这些方法适用于多种模型系统，并展示了它们在哺乳动物细胞和酿酒酵母中的应用实例。
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