Iturriza Gómara M, Simpson R, Perault A M, Redpath C, Lorgelly P, Joshi D, Mugford M, Hughes C A, Dalrymple J, Desselberger U, Gray J
Enteric Virus Unit, Virus Reference Department, Centre for Infections, Health Protection Agency, London, UK.
Epidemiol Infect. 2008 Jan;136(1):23-33. doi: 10.1017/S0950268807008059. Epub 2007 Feb 21.
The aim of this study was to investigate the burden of disease associated with gastroenteric viruses (rotavirus, norovirus, sapovirus, astrovirus and enteric adenovirus) using structured surveillance of children aged <6 years in the community. Faecal samples were collected between 2000 and 2003 from 685 children with symptoms of gastroenteritis. The children comprised three groups; 223 in the structured surveillance cohort, 203 in a community cohort and 259 in a cohort of hospitalized children. All samples were tested for the presence of viral pathogens using molecular methods. Questionnaires were sent to the parents/carers of the children recruited to the structured surveillance cohort in order to collect data that would allow an estimation of the severity of illness by means of the Vesikari score, and of the cost associated with gastrointestinal disease in this age group. A viral aetiological agent was detected in 53.5% of samples tested. Rotavirus was the most common pathogen found in all three cohorts followed by norovirus and enteric adenoviruses. Multiple viruses were found in 8% of the samples, and commonly involved rotavirus and any other virus. G1P[8] was the most commonly detected rotavirus strain and there was no significant difference in the distribution of rotavirus genotypes among the three cohorts. Analysis of the questionnaires indicated that rotavirus infections were likely to be more severe than any other virus infection, and children from whom a viral pathogen was identified were more likely to require rehydration therapy.
本研究的目的是通过对社区中6岁以下儿童进行结构化监测，调查与胃肠病毒（轮状病毒、诺如病毒、札如病毒、星状病毒和肠道腺病毒）相关的疾病负担。2000年至2003年间，从685名患有胃肠炎症状的儿童中采集了粪便样本。这些儿童分为三组：结构化监测队列中的223名、社区队列中的203名和住院儿童队列中的259名。使用分子方法对所有样本进行病毒病原体检测。向结构化监测队列中招募的儿童的父母/照顾者发送问卷，以便收集数据，从而通过韦西卡里评分估计疾病的严重程度，以及该年龄组胃肠道疾病的相关费用。在53.5%的检测样本中检测到病毒病原体。轮状病毒是在所有三个队列中发现的最常见病原体，其次是诺如病毒和肠道腺病毒。在8%的样本中发现了多种病毒，常见的是轮状病毒和任何其他病毒。G1P[8]是最常检测到的轮状病毒株，三个队列中轮状病毒基因型的分布没有显著差异。问卷分析表明，轮状病毒感染可能比任何其他病毒感染更严重，并且确定有病毒病原体的儿童更有可能需要补液治疗。
