Polat Zubeyde Akin, Vural Ayse, Tepe Bektas, Cetin Ali
CUTFAM, Cumhuriyet University, 58140, Sivas, Turkey.
Parasitol Res. 2007 Jul;101(2):397-402. doi: 10.1007/s00436-007-0487-x. Epub 2007 Feb 22.
Amoebic keratitis is difficult to treat without total efficacy in some patients because of cysts, which is less susceptible than trophozoites to the usual treatments. We investigated the in vitro effectiveness of the methanolic extract of four Allium species from Turkey against Acanthamoeba castellanii and its cytotoxicity on corneal cells in vitro. Extracts were evaluated for their amoebicidal activities using an inverted light microscope. The effect of the Allium species with concentrations ranging from 1.0 to 32.0 mg/ml on the proliferation of A. castellanii trophozoites and cysts were examined in vitro. For the determination of the cytotoxicity of the Allium species on corneal cells, agar diffusion tests were performed. According to the results obtained from the tests, A. scrodoprosum subsp. rotundum showed remarkable amoebicidal effect on A. castellanii, while the others remained inactive. In the case of cyctotoxic activities, the methanolic extracts of A. scrodoprosum showed no cytotoxicity for the cells in the concentration of 32 mg/ml, while the others exerted significant cytotoxic activities between the concentrations of 1 and 16 mg/ml. As a result, the methanolic extract of A. scrodoprosum could be concluded as a new natural agent against Acanthamoeba. On the other hand, it still needs to be further evaluated by in vivo test systems to confirm the efficiency of its biological effect.
由于囊肿的存在，阿米巴角膜炎在一些患者中难以完全有效治疗，囊肿比滋养体对常规治疗的敏感性更低。我们研究了来自土耳其的四种葱属植物的甲醇提取物对卡氏棘阿米巴的体外有效性及其对角膜细胞的体外细胞毒性。使用倒置光学显微镜评估提取物的杀阿米巴活性。在体外检查了浓度范围为1.0至32.0mg/ml的葱属植物对卡氏棘阿米巴滋养体和囊肿增殖的影响。为了测定葱属植物对角膜细胞的细胞毒性，进行了琼脂扩散试验。根据试验获得的结果，圆葱亚种对卡氏棘阿米巴表现出显著的杀阿米巴作用，而其他品种则无活性。在细胞毒性活性方面，圆葱的甲醇提取物在32mg/ml浓度下对细胞无细胞毒性，而其他品种在1至16mg/ml浓度之间表现出显著的细胞毒性活性。结果，圆葱的甲醇提取物可被认为是一种抗棘阿米巴的新型天然药物。另一方面，仍需要通过体内试验系统进一步评估，以确认其生物学效应的效率。
