Department of Parasitology, School of Medicine, Cumhuriyet University, 58140 Sivas, Turkey.
Parasitol Res. 2012 Feb;110(2):565-70. doi: 10.1007/s00436-011-2524-z. Epub 2011 Jul 7.
The aim of this study is to evaluate the in vitro amoebicidal activity of the aqueous extracts of Pastinaca armenea and Inula oculus-christi from Turkey. In the presence of aqueous extracts (ranging from 1.0 to 32.0 mg/ml), numbers of the viable Acanthamoeba castellanii trophozoites and cysts were decreased during the experimental process. Both extracts showed a time- and dose-dependent amoebicidal action on the trophozoites and cysts. Among the extracts tested, I. oculus showed the strongest amoebicidal effect on the trophozoites and cysts. In the presence of 32.00 mg/ml extract solution in the media, no viable trophozoites were determined from the time of 24 h. Moreover, in the presence of 16.00 mg/ml I. oculus-christi extract, no viable trophozoites were detected in 72 h of the experiment. Effectiveness of I. oculus-christi extract was found moderate against the cysts. In the presence of 32.00 mg/ml extract, only 25.3% of the total cysts were killed effectively. In the case of P. armenea, 40.3% and 23.0% of the trophozoites and cysts were killed in the presence of 32.00 mg/ml extract at the end of the experimental process (72 h), respectively.
本研究旨在评估来自土耳其的防风（Pastinaca armenea）和土木香（Inula oculus-christi）的水提物的体外杀阿米巴活性。在水提物（浓度范围为 1.0 至 32.0 mg/ml）存在的情况下，在实验过程中，可存活的棘阿米巴滋养体和包囊数量减少。两种提取物对滋养体和包囊均表现出时间和剂量依赖性的杀阿米巴作用。在所测试的提取物中，土木香对滋养体和包囊表现出最强的杀阿米巴作用。在含有 32.00 mg/ml 提取物的介质中，从 24 小时开始，没有确定存活的滋养体。此外，在存在 16.00 mg/ml 土木香提取物的情况下，在实验的 72 小时内没有检测到存活的滋养体。土木香提取物对包囊的有效性被发现为中等。在 32.00 mg/ml 提取物存在的情况下，只有 25.3%的总包囊被有效杀死。在防风的情况下，在实验结束时（72 小时），32.00 mg/ml 提取物分别杀死了 40.3%和 23.0%的滋养体和包囊。
