Department of Parasitology, School of Medicine, Cumhuriyet University, 58140, Sivas, Turkey.
Parasitol Res. 2012 Jun;110(6):2175-80. doi: 10.1007/s00436-011-2744-2. Epub 2011 Dec 9.
Amoebic keratitis is difficult to treat without total efficacy in some patients because of cysts, which are less susceptible than trophozoites to the usual treatments. The aim of this study is to evaluate the in vitro amoebicidal activity of the methanolic extracts of Satureja cuneifolia and Melissa officinalis. In the presence of methanolic extracts (ranging from 1.0 to 32.0 mg/ml), numbers of the viable Acanthamoe castellanii trophozoites and cysts were decreased during the experimental process. Both extracts showed a time- and dose-dependent amoebicidal action on the trophozoites and cysts. Among the extracts tested, S. cuneifolia showed the strongest amoebicidal effect on the trophozoites and cysts. In the presence of 32 mg/ml extract, no viable trophozoites were observed within 24 h. At the same concentration value, the extract was found effective against the cysts at a rate of 46.3% within 72 h of the experimental process. At 16 mg/ml extract concentration, no viable trophozoites were also observed in the 24th hour of the experiment. At the end of the experimental process, 34.7% of the cysts were killed by the extract. M. officinalis showed moderate amoebicidal effect. At the concentration of 32 mg/ml, 44.3% and 30.0% of the trophozoites and cysts were killed by the extract, respectively. Results obtained from these concentration values were found statistically different in terms of their actions both on trophozoites and cysts (p<0.05).
棘阿米巴角膜炎在某些患者中由于包囊的存在而难以获得完全疗效，因为包囊比滋养体对常规治疗的敏感性差。本研究旨在评估 Satureja cuneifolia 和 Melissa officinalis 的甲醇提取物的体外杀阿米巴活性。在甲醇提取物（浓度范围为 1.0 至 32.0 mg/ml）的存在下，实验过程中活 Acanthamoe castellanii 滋养体和包囊的数量减少。两种提取物对滋养体和包囊均表现出时间和剂量依赖性的杀阿米巴作用。在所测试的提取物中，S. cuneifolia 对滋养体和包囊表现出最强的杀阿米巴作用。在 32 mg/ml 提取物存在下，在 24 小时内未观察到活的滋养体。在相同的浓度值下，提取物在 72 小时的实验过程中对包囊的有效率为 46.3%。在 16 mg/ml 提取物浓度下，在实验的第 24 小时也未观察到活的滋养体。在实验结束时，34.7%的包囊被提取物杀死。M. officinalis 表现出中等的杀阿米巴作用。在 32 mg/ml 的浓度下，提取物分别杀死了 44.3%和 30.0%的滋养体和包囊。从这些浓度值获得的结果在其对滋养体和包囊的作用方面均具有统计学差异（p<0.05）。
