Långström Bengt, Andrén Per E, Lindhe Orjan, Svedberg Marie, Hall Håkan
Department of Biochemistry and Organic Chemistry, Uppsala University, Uppsala, Sweden.
Mol Imaging Biol. 2007 Jul-Aug;9(4):161-75. doi: 10.1007/s11307-007-0088-1.
Neurodegeneration induces various changes in the brain, changes that may be investigated using neuroimaging techniques. The in vivo techniques are useful for the visualization of major changes, and the progressing abnormalities may also be followed longitudinally. However, to study and quantify minor abnormalities, neuroimaging of postmortem brain tissue is used. These in vitro methods are complementary to the in vivo techniques and contribute to the knowledge of pathophysiology and etiology of the neurodegenerative diseases. In vitro radioligand autoradiography has given great insight in the involvement of different neuronal receptor systems in these diseases. Data on the dopamine and cholinergic systems in neurodegeneration are discussed in this review. Also, the amyloid plaques are studied using in vitro radioligand autoradiography. Using one of the newer methods, imaging matrix-assisted laser desorption ionization mass spectrometry, the distribution of a large number of peptides and proteins may be detected in vitro on brain cryosections. In this overview, we describe in vitro imaging techniques in the neurodegenerative diseases as a complement to in vivo positron emission tomography and single photon emission computed tomography imaging.
神经退行性变会在大脑中引发各种变化，这些变化可用神经成像技术进行研究。活体技术有助于观察主要变化，而且进展中的异常情况也可进行纵向跟踪。然而，为了研究和量化微小异常，会采用死后脑组织的神经成像。这些体外方法是对活体技术的补充，有助于了解神经退行性疾病的病理生理学和病因。体外放射性配体自显影术让我们深入了解了不同神经元受体系统在这些疾病中的作用。本综述讨论了神经退行性变中多巴胺和胆碱能系统的数据。此外，还使用体外放射性配体自显影术对淀粉样斑块进行研究。使用更新的方法之一，即成像基质辅助激光解吸电离质谱法，可以在脑冷冻切片上体外检测大量肽和蛋白质的分布。在本综述中，我们将神经退行性疾病中的体外成像技术描述为对活体正电子发射断层扫描和单光子发射计算机断层扫描成像的补充。
