Llana-Ruiz-Cabello María, Gutiérrez-Praena Daniel, Puerto María, Pichardo Silvia, Jos Ángeles, Cameán Ana María
Area of Toxicology, Faculty of Pharmacy, University of Sevilla, Profesor García González 2, 41012 Sevilla, Spain.
Area of Toxicology, Faculty of Pharmacy, University of Sevilla, Profesor García González 2, 41012 Sevilla, Spain.
Toxicol In Vitro. 2015 Jun;29(4):647-56. doi: 10.1016/j.tiv.2015.02.006. Epub 2015 Feb 21.
The food industry needs to provide consumers with fresh and healthy products. In this context, food packaging plays an important role. Thus, certain essential oils are being incorporated into plastic polymers to confer better preservative properties. The oregano essential oil contains carvacrol and thymol, two important polyphenols. Considering their increasing use in active food packaging, the evaluation of their suitability and safety is of great interest. In the present work, a concentration-dependent increase in the antioxidant effects of carvacrol, thymol, and their mixture (10:1) was determined using DPPH and ABTS assays. In addition, the safety of these compounds was tested in vitro. Reactive oxygen species and glutathione levels were measured after exposing cells for 24 and 48 h to different concentrations of carvacrol, thymol and their mixture. The abilities of these compounds to protect against or revert the effects of H2O2 on cells were also studied. The results showed that oxidative stress plays a role in the damage induced by carvacrol and the mixture at high concentrations. However, at lower concentrations, both compounds and their mixture were shown, for the first time, to protect cells against the damage induced by the H2O2.
食品行业需要向消费者提供新鲜且健康的产品。在此背景下，食品包装发挥着重要作用。因此，某些精油正被添加到塑料聚合物中以赋予更好的防腐性能。牛至精油含有香芹酚和百里香酚这两种重要的多酚类物质。鉴于它们在活性食品包装中的使用日益增加，对其适用性和安全性的评估备受关注。在本研究中，使用DPPH和ABTS法测定了香芹酚、百里香酚及其混合物（10:1）抗氧化作用的浓度依赖性增加。此外，还对这些化合物进行了体外安全性测试。将细胞暴露于不同浓度的香芹酚、百里香酚及其混合物24小时和48小时后，测量了活性氧和谷胱甘肽水平。还研究了这些化合物预防或逆转过氧化氢对细胞影响的能力。结果表明，氧化应激在高浓度香芹酚及其混合物诱导的损伤中起作用。然而，在较低浓度下，首次表明这两种化合物及其混合物可保护细胞免受过氧化氢诱导的损伤。
