Linne von Berg K H, Kowallik K V
Institut für Botanik, Heinrich-Heine-Universität, Düsseldorf, FRG.
Plant Mol Biol. 1992 Jan;18(1):83-95. doi: 10.1007/BF00018459.
The chloroplast genome of the chromophytic alga Vaucheria bursata has been characterized by restriction site and gene mapping analysis. It is represented by a circular molecule 124.6 kb in size. An inverted sequence duplication (IR) not larger than 5.85 kb carries the rRNA genes and separates two single-copy regions of 64.6 kb and 48.3 kb from one another. The Vaucheria plastid genome exists in two equimolar isomers which is due to intramolecular flip-flop recombination within the IR sequences. The coding sites for 21 structural and soluble proteins have been mapped on both single-copy regions using heterologous gene sequences as probes. Although the overall gene order is found to be rearranged when compared with other chromophytic algal and land plant chloroplast genomes, most of the transcriptional units of cyanobacteria and land plant chloroplast genomes appear to be conserved. The phylogenetic implications of these findings are further discussed.
褐藻囊链藻的叶绿体基因组已通过限制性酶切位点和基因图谱分析进行了表征。它由一个大小为124.6 kb的环状分子代表。一个不大于5.85 kb的反向序列重复（IR）携带rRNA基因，并将两个大小分别为64.6 kb和48.3 kb的单拷贝区域彼此分开。囊链藻质体基因组以两种等摩尔异构体形式存在，这是由于IR序列内的分子内翻转重组所致。使用异源基因序列作为探针，已在两个单拷贝区域上绘制了21种结构和可溶性蛋白质的编码位点。尽管与其他褐藻和陆地植物叶绿体基因组相比，整体基因顺序发生了重排，但蓝细菌和陆地植物叶绿体基因组的大多数转录单元似乎是保守的。这些发现的系统发育意义将进一步讨论。
