Botanisches Institut der Universität Düsseldorf, Universitätsstraße 1, D-4000, Düsseldorf, FRG.
Plant Mol Biol. 1985 Nov;4(6):365-76. doi: 10.1007/BF02418258.
Plastids of the brown algaDictyota dichotoma contain a single homogeneous DNA species which bands at a buoyant density of 1.693 g/cm(3) in neutral CsCl equilibrium density gradients. The corresponding nuclear DNA has a density of 1.715 g/cm(3). The molecular size of the plastid DNA is 123 kbp as calculated by both electron microscopy of spread intact circular molecules and gel electrophoresis following single and double digestions with various restriction enzymes. A restriction map has been constructed using the endonucleases Sal I, Bam HI, and Bgl II which cleave theDictyota plastome into 6, 12, and 17 fragments, respectively. No large repeated regions, as found in chlorophycean andEuglena plastid DNAs, were detected.Dictyota dichotoma is the first member from the chlorophyll c-line of the algal pedigree for which a physical map of plastid DNA has been established.
褐藻 Dictyota dichotoma 的质体含有一种单一的同质 DNA 物种，在中性 CsCl 平衡密度梯度中以 1.693 g/cm³的浮力密度带型出现。相应的核 DNA 密度为 1.715 g/cm³。通过电子显微镜观察展开的完整圆形分子以及用各种限制酶进行单酶切和双酶切后的凝胶电泳，分别计算出质体 DNA 的分子大小为 123 kbp。使用内切酶 Sal I、Bam HI 和 Bgl II 构建了一个限制图谱，它们分别将 Dictyota 质体切割成 6、12 和 17 个片段。与叶绿素 c 系藻类和 Euglena 质体 DNA 中发现的大重复区域不同，未检测到 Dictyota dichotoma 中的大重复区域。Dictyota dichotoma 是第一个建立质体 DNA 物理图谱的藻类血统的叶绿素 c 系成员。
