Tippetts M T, Robertson D L, Smith M A
Graduate Section of Biochemistry, Brigham Young University, Provo, Utah 84602.
Mol Cell Biochem. 1991 Jan 16;100(1):61-70. doi: 10.1007/BF00230810.
A recombinant DNA library was constructed from partial BamHI or MboI digests of safflower (Carthamus tinctorius L.) chloroplast DNA, in the BamHI site of lambda EMBL3. Seventeen lambda recombinants, selected by chromosome walking, were found to contain overlapping fragments of the entire chloroplast genome. These clones were mapped using single and double digests of BamHI, EcoRI and HindIII. cDNAs synthesized from isolated 16S and 23S chloroplast rRNAs were used to map the ribosomal RNA genes relative to physical maps of the above restriction enzymes. The mapped positions of the rRNA genes for the safflower chloroplast DNA are in good agreement with previously published data for tobacco, spinach and several other higher plants.
用红花（Carthamus tinctorius L.）叶绿体DNA的部分BamHI或MboI酶切片段，在λEMBL3的BamHI位点构建了一个重组DNA文库。通过染色体步移选择出17个λ重组体，发现它们包含整个叶绿体基因组的重叠片段。使用BamHI、EcoRI和HindIII的单酶切和双酶切对这些克隆进行图谱绘制。从分离的16S和23S叶绿体rRNA合成的cDNA用于相对于上述限制酶的物理图谱定位核糖体RNA基因。红花叶绿体DNA的rRNA基因的定位与先前发表的烟草、菠菜和其他几种高等植物的数据高度一致。
