十字花科中降解硫代葡萄糖苷的酶——黑芥子酶由一个基因家族编码。
The glucosinolate-degrading enzyme myrosinase in Brassicaceae is encoded by a gene family.
作者信息
Xue J P, Lenman M, Falk A, Rask L
机构信息
Department of Cell Research, Swedish University of Agricultural Sciences, Uppsala Genetic Center.
出版信息
Plant Mol Biol. 1992 Jan;18(2):387-98. doi: 10.1007/BF00034965.
A full-length cDNA clone (MB3) and three partial clones (MA1, MB1 and MB2) which encode myrosinase (thioglucoside glucohydrolase, EC 3.2.3.1) were isolated from a Sinapis alba (white mustard) cDNA library. Nucleotide sequence analysis of these clones revealed that they are encoded by a gene family. Southern blot analysis with gene-specific probes showed that the gene family consists of a least two subfamilies (MA and MB) each with several members both in S. alba and in Brassica napus (oilseed rape). In Arabidopsis thaliana (wall cress) only three myrosinase genes seem to be present. Northern blot analysis indicated that all the myrosinase mRNA species have the same size, approximately 1.95 kb.
从白芥（Sinapis alba）cDNA文库中分离出一个编码黑芥子酶（硫代葡萄糖苷葡萄糖水解酶，EC 3.2.3.1）的全长cDNA克隆（MB3）和三个部分克隆（MA1、MB1和MB2）。对这些克隆进行核苷酸序列分析表明，它们由一个基因家族编码。用基因特异性探针进行的Southern印迹分析表明，该基因家族至少由两个亚家族（MA和MB）组成，在白芥和甘蓝型油菜（油菜籽）中每个亚家族都有几个成员。在拟南芥（鼠耳芥）中似乎仅存在三个黑芥子酶基因。Northern印迹分析表明，所有黑芥子酶mRNA种类大小相同，约为1.95 kb。
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