Woo Yin Ling, Damay Isabelle, Stanley Margaret, Crawford Robin, Sterling Jane
Division of Virology, Department of Pathology, University of Cambridge, CB2 2QQ Cambridge, UK.
J Virol Methods. 2007 Jun;142(1-2):226-30. doi: 10.1016/j.jviromet.2007.01.029. Epub 2007 Feb 23.
Accurate HPV typing is important for natural history and epidemiology studies. With the introduction of prophylactic multivalent HPV vaccines, there is also the need to determine the dominant genotypes in different populations and the effect of a vaccination programme on infection profiles. The interplay between multiple infection, viral persistence and implementation of interventions is a complicated one and therefore requires a reliable and accurate HPV detection and typing method. The Linear Array HPV genotyping test is a PCR-based HPV detection kit which can detect qualitatively Multiple HPV Infection in cervical cells collected in PreservCyt Solution. The utility of this kit for multiple HPV typing of archival frozen tissue and cervical cells not collected in PreservCyt are described.
准确的人乳头瘤病毒（HPV）分型对于自然史和流行病学研究很重要。随着预防性多价HPV疫苗的引入，还需要确定不同人群中的主要基因型以及疫苗接种计划对感染谱的影响。多重感染、病毒持续存在和干预措施实施之间的相互作用很复杂，因此需要一种可靠且准确的HPV检测和分型方法。线性阵列HPV基因分型检测是一种基于聚合酶链反应（PCR）的HPV检测试剂盒，可定性检测保存在PreservCyt溶液中的宫颈细胞中的多重HPV感染。本文描述了该试剂盒在对存档冷冻组织和未保存在PreservCyt中的宫颈细胞进行多重HPV分型方面的应用。
