Lawson John D, Bridges William C, Adelberg Jeffrey W
Department of Plant and Environmental Sciences, Clemson University, Clemson, SC 29634, USA.
Department of Mathematics, Clemson University, Clemson, SC 29634, USA.
Plants (Basel). 2023 Jan 7;12(2):289. doi: 10.3390/plants12020289.
Difficult-to-root plants often perform poorly during acclimatization and in vitro rooting can increase the survival and quality of plants. The influence of auxin application and mineral nutrition on in vitro rooting and subsequent effects on plant quality in eight genotypes were investigated. Microshoots were rooted in vitro on Murashige and Skoog (MS), ½ MS, Driver and Kuniyuki (DKW), or New Prunus Medium (NPM) media formulations in combination with 15 µM indole-3-butyric acid (IBA), 4-day 15 µM IBA pulse, 1 mM 30 s quick-dip, or IBA-free treatments. Shoots were observed pre- and post-acclimatization to determine rooting methods to maximize quality and minimize labor. A genotype-specific response to auxin application was observed with seven of eight genotypes achieving 100% survival when paired with the recommended IBA treatment. Peaches performed best when treated with 4-day IBA pulse or 30 s quick-dip. Rooting of , it's hybrid to and all benefitted from IBA application. Shoots rooted with 15 µM IBA were smaller and lower quality in most genotypes. DKW maximized size and quality in six genotypes. Better shoots and larger root systems during in vitro rooting produced better plants in the greenhouse with no detrimental effect of callus growth. Rooting techniques to maximize plant quality while reducing labor are specified.
难生根植物在驯化过程中往往表现不佳，而离体生根可以提高植物的存活率和质量。研究了生长素施用和矿质营养对8个基因型植物离体生根的影响以及对后续植物质量的影响。将微枝在添加15 μM吲哚-3-丁酸（IBA）、4天15 μM IBA脉冲处理、1 mM 30秒速蘸处理或无IBA处理的Murashige和Skoog（MS）、1/2 MS、Driver和Kuniyuki（DKW）或新李属培养基（NPM）配方上进行离体生根。在驯化前后观察嫩枝，以确定能使质量最大化并减少劳动力的生根方法。观察到不同基因型对生长素施用有特异性反应，8个基因型中有7个与推荐的IBA处理配对时存活率达到100%。桃用4天IBA脉冲处理或30秒速蘸处理时表现最佳。它与 、 的杂交种生根均受益于IBA施用。在大多数基因型中，用15 μM IBA生根的嫩枝较小且质量较低。DKW使6个基因型的嫩枝大小和质量最大化。离体生根过程中更好的嫩枝和更大的根系在温室中能培育出更好的植株，且对愈伤组织生长没有不利影响。明确了能在提高植物质量的同时减少劳动力的生根技术。
