Barocchi Michèle Anne, Censini Stefano, Rappuoli Rino
Novartis Vaccines, Via Fiorentina, 53100 Siena, Italy.
Vaccine. 2007 Apr 20;25(16):2963-73. doi: 10.1016/j.vaccine.2007.01.065. Epub 2007 Jan 30.
In this review we aim to provide the reader with an understanding of the capsular-based complexity of Streptococcus pneumoniae, one of the main limitations to current vaccine development. We then discuss the need for a new vaccine strategy based on proteic antigen candidates discovered in silico. Describing specifically how reverse vaccinology coupled to conventional vaccinology has led to a new paradigm of vaccine development. Finally, we conclude with the importance of defining the pan-genome of the pneumococcus, that is, the sequencing and analysis of multiple genomes from the same species. A critical factor in determining conserved proteins in a group of epidemiologically relevant circulating S. pneumoniae strains, in order to achieve the greatest coverage. Ultimately, the identification of immunogenic surface antigens and assessment of their efficacy will be imperative in the development of a vaccine with the ability to protect against invasive disease independent of serotype.
在本综述中，我们旨在让读者了解肺炎链球菌基于荚膜的复杂性，这是当前疫苗研发的主要限制因素之一。然后，我们讨论了基于通过计算机发现的蛋白质抗原候选物制定新疫苗策略的必要性。具体描述了反向疫苗学与传统疫苗学相结合如何引领了疫苗研发的新范式。最后，我们总结了定义肺炎球菌泛基因组的重要性，即对同一物种的多个基因组进行测序和分析。这是确定一组具有流行病学相关性的循环肺炎链球菌菌株中保守蛋白的关键因素，以便实现最大覆盖率。最终，鉴定免疫原性表面抗原并评估其功效对于开发能够预防侵袭性疾病且不受血清型影响的疫苗至关重要。
