Carnovale S, Lorenzo J, Kaufman S, Finquelievich J, Guelfand L
Centro de Micología, Dep de Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay.
Med Mycol. 2007 Feb;45(1):51-6. doi: 10.1080/13693780601028675.
Trichosporon asahii is an opportunistic fungus considered the leading etiologic agent of trichospornosis, a disease that causes great morbidity/mortality among affected patients. The identification of the etiologic agent is generally obtained through physiological and morphological studies. Molecular investigations, such as species-specific primers (PCR), have recently been developed with the aim of applying a more simple, specific, and faster technology for mycological diagnosis. The genetic material amplification technique using ad-random primers (RAPD: random amplified polymorphic DNA) is an epidemiological tool which enables research on infection by and transmission of suspected agents. In this study, the amplified polymorphic DNA technique was used to determine the intraspecific diversity of 10 Trichosporon asahii strains. Primers OPAO-15 and 1821 were used and these allowed association to 5 and 3 electrophoretic patterns, respectively. The T. asahii molecular identification, which had been previously analyzed by conventional methods, was performed by means of primers TAAF and pITS4. Our results support the use of these techniques for clonality studies of the strains of this fungus as well as for the fast and specific identification of its members in clinical cases.
白吉利丝孢酵母是一种机会性真菌，被认为是毛孢子菌病的主要病原体，这种疾病在受影响的患者中会导致很高的发病率/死亡率。病原体的鉴定通常通过生理和形态学研究来进行。分子研究，如种特异性引物（PCR），最近已经开发出来，旨在应用一种更简单、特异且快速的技术进行真菌学诊断。使用随机引物的遗传物质扩增技术（RAPD：随机扩增多态性DNA）是一种流行病学工具，可用于研究疑似病原体的感染和传播。在本研究中，扩增的多态性DNA技术用于确定10株白吉利丝孢酵母菌株的种内多样性。使用了引物OPAO - 15和1821，它们分别与5种和3种电泳图谱相关联。先前通过传统方法分析的白吉利丝孢酵母分子鉴定，通过引物TAAF和pITS4进行。我们的结果支持使用这些技术进行该真菌菌株的克隆性研究以及在临床病例中对其成员进行快速和特异性鉴定。
