Smith F L, Carper S W, Hall J S, Gilligan B J, Madsen E L, Storm F K
Division of Urology, University of Wisconsin School of Medicine, Madison.
J Urol. 1992 Feb;147(2):486-90. doi: 10.1016/s0022-5347(17)37285-3.
High energy shock waves produced by a piezoelectric lithotripter, (EDAP LT.01) and an electrohydraulic lithotripter, (Dornier HM3) were examined for their effects on Chinese hamster ovary cells in suspension. The EDAP caused acute lactate dehydrogenase release, consistent with severe membrane disruption in a proportion of cells, with the remaining proportion of cells replicating normally as measured by clonogenic assay. Similarly, the Dornier also caused lactate dehydrogenase release. However, a significant proportion of cells which remained "viable" after Dornier treatment, (intact to lactate dehydrogenase), did not replicate by clonogenic assay. The Dornier HM3 lithotripter has been reported to produce free radicals in aqueous solution. In the current investigation, we could not detect significant free radical formation from the EDAP LT.01. Chinese hamster ovary cell killing by the Dornier HM3 was significantly augmented by radiosensitizers, 5-iodo-2-deoxyuridine or buthionine sulfoximine, while radioprotectors cysteamine and WR-1065 had no protective effect. EDAP cell killing was not influenced by either radioprotectors or radiosensitizers. The mechanism of in vitro cytotoxicity differs between piezoelectric and electrohydraulic high energy shock wave delivery.
研究了压电碎石机（EDAP LT.01）和液电碎石机（Dornier HM3）产生的高能冲击波对悬浮培养的中国仓鼠卵巢细胞的影响。EDAP导致急性乳酸脱氢酶释放，这与部分细胞的严重膜破坏一致，通过克隆形成试验检测，其余部分细胞正常复制。同样，Dornier也导致乳酸脱氢酶释放。然而，Dornier处理后仍“存活”（对乳酸脱氢酶完整）的相当一部分细胞，通过克隆形成试验无法复制。据报道，Dornier HM3碎石机在水溶液中会产生自由基。在当前研究中，我们未检测到EDAP LT.01产生明显的自由基形成。放射增敏剂5-碘-2-脱氧尿苷或丁硫氨酸亚砜胺可显著增强Dornier HM3对中国仓鼠卵巢细胞的杀伤作用，而放射保护剂半胱胺和WR-1065则无保护作用。EDAP对细胞的杀伤作用不受放射保护剂或放射增敏剂的影响。压电和液电高能冲击波传递的体外细胞毒性机制不同。
