Weisblum B, Demohn V
J Bacteriol. 1970 Mar;101(3):1073-5. doi: 10.1128/jb.101.3.1073-1075.1970.
Thiostrepton inhibits (14)C-leucine incorporation by intact cells of Bacillus megaterium as well as (14)C-phenylalanine incorporation by a poly U-directed extract of Escherichia coli. Extracts of E. coli which are pretreated by incubation with thiostrepton cannot be reactivated by dialysis to more than 5% of their former activity. The 50S ribosome subunit appears to be the site of thiostrepton action, since protein-synthesizing activity can be restored to dialyzed pretreated extracts by supplementation with 50S ribosome subunits but not with 30S ribosome subunits. This technique also provides a simple sensitive method for detection of the biological activity of very small amounts of 50S ribosome subunits.
硫链丝菌素抑制巨大芽孢杆菌完整细胞对（14）C - 亮氨酸的掺入以及大肠杆菌多聚U指导提取物对（14）C - 苯丙氨酸的掺入。用硫链丝菌素孵育预处理过的大肠杆菌提取物，通过透析不能重新激活至其先前活性的5%以上。50S核糖体亚基似乎是硫链丝菌素的作用位点，因为通过补充50S核糖体亚基而非30S核糖体亚基，可以使透析后的预处理提取物恢复蛋白质合成活性。该技术还提供了一种简单灵敏的方法来检测极少量50S核糖体亚基的生物活性。
