大鼠肝癌中甘油激酶和3-磷酸甘油脱氢酶的比例活性。
Proportional activities of glycerol kinase and glycerol 3-phosphate dehydrogenase in rat hepatomas.
作者信息
Harding J W, Pyeritz E A, Morris H P, White H B
出版信息
Biochem J. 1975 Jun;148(3):545-50. doi: 10.1042/bj1480545.
The activities of glycerol 3-phosphate dehydrogenase (EC 1.1.1.8), glycerol kinase (EC 2.7.1.30), lactate dehydrogenase (EC 1.1.1.27), "malic' enzyme (L-malate-NADP+ oxidoreductase; EC 1.1.1.40) and the beta-oxoacyl-(acyl-carrier protein) reductase component of the fatty acid synthetase complex were measured in nine hepatoma lines (8 in rats, 1 in mouse) and in the livers of host animals. With the single exception of Morris hepatoma 16, which had unusually high glycerol 3-phosphate dehydrogenase activity, the activities of glycerol 3-phosphate dehydrogenase and glycerol kinase were highly correlated in normal livers and hepatomas (r = 0.97; P less than 0.01). The activities of these two enzymes were not strongly correlated with the activities of any of the other three enzymes. The primary function of hepatic glycerol 3-phosphate dehydrogenase appears to be in gluconeogenesis from glycerol.
在九条肝癌细胞系（8条大鼠肝癌细胞系，1条小鼠肝癌细胞系）以及宿主动物的肝脏中，对3-磷酸甘油脱氢酶（EC 1.1.1.8）、甘油激酶（EC 2.7.1.30）、乳酸脱氢酶（EC 1.1.1.27）、“苹果酸”酶（L-苹果酸-NADP+氧化还原酶；EC 1.1.1.40）以及脂肪酸合成酶复合体的β-氧代酰基-（酰基载体蛋白）还原酶组分的活性进行了测定。除了3-磷酸甘油脱氢酶活性异常高的莫里斯肝癌16细胞系外，正常肝脏和肝癌组织中3-磷酸甘油脱氢酶和甘油激酶的活性高度相关（r = 0.97；P < 0.01）。这两种酶的活性与其他三种酶中的任何一种的活性均无强烈相关性。肝脏中3-磷酸甘油脱氢酶的主要功能似乎在于由甘油进行糖异生作用。
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