甘油-3-磷酸脱氢酶在甘油酯代谢中的作用。日粮对鸡肝脏中酶活性的影响。

Role of glycerol 3-phosphate dehydrogenase in glyceride metabolism. Effect of diet on enzyme activities in chicken liver.

作者信息

Harding J W, Pyeritz E A, Copeland E S, White H B

出版信息

Biochem J. 1975 Jan;146(1):223-9. doi: 10.1042/bj1460223.

DOI:10.1042/bj1460223

PMID:167714

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1165291/

Abstract

The metabolic role of hepatic NAD-linked glycerol 3-phosphate dehydrogenase (EC 1.1.1.8) was investigated vis-a-vis glyceride synthesis, glyceride degradation and the maintainence of the NAD redox state. 2. Five-week-old chickens were placed on five dietary regimes: a control group, a group on an increased-carbohydrate-lowered-fat diet, a group on a high-fat-lowered-carbohydrate diet, a starved group and a starved-refed group. In each group the specific activity (mumol/min per g wet wt. of tissue) of hepatic glycerol 3-phosphate dehydrogenase was compared with the activities of the beta-oxoacyl-(acyl-carrier protein) reductase component of fatty acid synthetase, glycerol kinase (EC 2.7.1.30) and lactate dehydrogenase (EC 1.1.1.27). 3. During starvation, the activities of glycerol 3-phosphate dehydrogenase, glycerol kinase and lactate dehydrogenase rose significantly. After re-feeding these activities returned to near normal. All three activities rose slightly on the high-fat diet. Lactate dehydrogenase activity rose slightly, whereas those of the other two enzymes fell slightly on the increased-carbohydrate-lowered-fat diet. 4. The activity of the beta-oxoacyl-(acyl-carrier protein) reductase component of fatty acid synthetase, a lipid-synthesizing enzyme, contrasted strikingly with the other three enzyme activities. Its activity was slightly elevated on the increased-carbohydrate diet and significantly diminished on the high-fat diet and during starvation. 5. The changes in activity of the chicken liver isoenzyme of glycerol 3-phosphate dehydrogenase in response to dietary stresses suggest that the enzyme has an important metabolic role other than or in addition to glyceride biosynthesis.

摘要

针对甘油酯合成、甘油酯降解以及NAD氧化还原状态的维持，对肝脏中与NAD相关的甘油-3-磷酸脱氢酶（EC 1.1.1.8）的代谢作用进行了研究。2. 将五周龄的鸡分为五组饮食方案：一组为对照组，一组为高碳水化合物低脂肪饮食组，一组为高脂肪低碳水化合物饮食组，一组为饥饿组，一组为饥饿再喂食组。比较每组肝脏甘油-3-磷酸脱氢酶的比活性（每克组织湿重每分钟微摩尔数）与脂肪酸合成酶的β-氧代酰基-（酰基载体蛋白）还原酶组分、甘油激酶（EC 2.7.1.30）和乳酸脱氢酶（EC 1.1.1.27）的活性。3. 在饥饿期间，甘油-3-磷酸脱氢酶、甘油激酶和乳酸脱氢酶的活性显著升高。再喂食后，这些活性恢复到接近正常水平。在高脂肪饮食时，这三种酶的活性均略有升高。在高碳水化合物低脂肪饮食时，乳酸脱氢酶活性略有升高，而其他两种酶的活性略有下降。4. 脂肪酸合成酶的β-氧代酰基-（酰基载体蛋白）还原酶组分（一种脂质合成酶）的活性与其他三种酶的活性形成了显著对比。其活性在高碳水化合物饮食时略有升高，在高脂肪饮食和饥饿期间显著降低。5. 鸡肝脏甘油-3-磷酸脱氢酶同工酶活性对饮食应激的变化表明，该酶除了甘油酯生物合成之外或之外还具有重要的代谢作用。