Sadava D, Depper M, Gilbert M, Bernard B, McCabe E R
Biol Neonate. 1987;52(1):26-32. doi: 10.1159/000242681.
The activities of three key enzymes of glycerol metabolism were measured in liver samples from 37 human fetuses ranging in gestational age from 18 weeks to term, from neonates (1-3 days) and from infants to 2 years. Glycerol kinase specific activity was constant throughout the period of fetal development examined, and was comparable to that measured in neonates and infants. However, the subcellular distribution of the activity changed markedly, being predominantly particulate in fetal samples and cytoplasmic in postnatal samples. The particulate activity had an elevated Km for glycerol. Cytoplasmic glycerol-3-phosphate dehydrogenase activity was very low in the fetal period, and then rose to adult levels during infancy. There were no kinetic differences between the fetal and postnatal activities. Mitochondrial glycerol-3-phosphate dehydrogenase activity rose somewhat after birth to near adult levels. The data indicate that glycerol can be metabolized by human fetal, neonatal and infant liver.
在取自37例胎龄从18周直至足月的人类胎儿、新生儿（1 - 3日龄）以及2岁以下婴幼儿的肝脏样本中，测定了甘油代谢的三种关键酶的活性。在所研究的胎儿发育阶段，甘油激酶的比活性保持恒定，且与在新生儿和婴幼儿中测得的活性相当。然而，该活性的亚细胞分布发生了显著变化，在胎儿样本中主要为颗粒状，而在出生后样本中为细胞质状。颗粒状活性的甘油米氏常数升高。胎儿期细胞质甘油 - 3 - 磷酸脱氢酶活性非常低，然后在婴儿期升至成人水平。胎儿期和出生后活性之间没有动力学差异。线粒体甘油 - 3 - 磷酸脱氢酶活性在出生后有所上升，接近成人水平。数据表明，甘油可被人类胎儿、新生儿及婴幼儿的肝脏代谢。
