College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China.
Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4380-4385. doi: 10.1099/ijs.0.050864-0. Epub 2013 Jul 5.
Two strictly aerobic, extremely halophilic archaea, strains WSY15-H1(T) and WSY15-H3(T), were isolated from a salt mine in Wensu county, Xinjiang province, China. Cells of the two strains were Gram-stain-negative, non-motile and pleomorphic. Colonies were pink- and red-pigmented, respectively. Strain WSY15-H1(T) grew at 20-45 °C (optimum 37-42 °C), 1.6-5.4 M NaCl (optimum 3.4-3.9 M), 0-2.0 M MgCl2 (optimum 0.1-0.5 M) and pH 6.0-9.0 (optimum 7.0), whereas strain WSY15-H3(T) grew at 20-50 °C (optimum 37 °C), 1.9-5.4 M NaCl (optimum 3.4 M), 0.02-2.5 M MgCl2 (optimum 0.5-1.0 M) and pH 6.0-7.5 (optimum 6.5). The minimal NaCl concentrations to prevent cell lysis were 9 % (w/v) for strain WSY15-H1(T) and 8 % (w/v) for strain WSY15-H3(T). The major polar lipids of the two isolates were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and phosphatidylglycerol sulfate, as well as nine glycolipids for strain WSY15-H1(T) and seven glycolipids for strain WSY15-H3(T); two of these glycolipids (GL1 and GL3) were chromatographically identical to bis-sulfated diglycosyl diether (S2-DGD-1) and sulfated diglycosyl diether (S-DGD-1), respectively. The genomic DNA G+C contents of strains WSY15-H1(T) and WSY15-H3(T) were 65.4 and 66.2 mol%. On the basis of 16S rRNA gene sequence analysis, strains WSY15-H1(T) and WSY15-H3(T) shared 97.0% similarity with each other and showed respectively 98.4 and 97.6% sequence similarity to Halolamina pelagica TBN21(T), which was the only type strain that had higher than 91% sequence similarity with the two isolates. Analysis of phylogenetic relationships and DNA-DNA relatedness indicated that strains WSY15-H1(T) and WSY15-H3(T) represent two novel lineages with closest affinity to H. pelagica TBN21(T). Based on phenotypic, chemotaxonomic and genotypic characteristics, two novel species of the genus Halolamina are proposed, Halolamina salifodinae sp. nov. (type strain WSY15-H1(T) = JCM 18548(T) = GCMCC 1.12371(T)) and Halolamina salina sp. nov. (type strain WSY15-H3(T) = JCM 18549(T) = GCMCC 1.12285(T)).
两株严格好氧、极端嗜盐的古菌，WSY15-H1(T)和 WSY15-H3(T)，从中国新疆温泉县的一个盐矿中分离得到。两株菌的细胞革兰氏染色阴性，不运动，形态多样。菌落分别呈粉红色和红色。菌株 WSY15-H1(T)在 20-45°C（最佳 37-42°C）、1.6-5.4 M NaCl（最佳 3.4-3.9 M）、0-2.0 M MgCl2（最佳 0.1-0.5 M）和 pH 6.0-9.0（最佳 7.0）下生长，而菌株 WSY15-H3(T)在 20-50°C（最佳 37°C）、1.9-5.4 M NaCl（最佳 3.4 M）、0.02-2.5 M MgCl2（最佳 0.5-1.0 M）和 pH 6.0-7.5（最佳 6.5）下生长。防止细胞裂解的最小 NaCl 浓度分别为 9%（w/v）用于菌株 WSY15-H1(T)和 8%（w/v）用于菌株 WSY15-H3(T)。两种分离物的主要极性脂为磷脂酰甘油、磷酸甘油磷酸甲酯和磷脂酰甘油硫酸酯，以及菌株 WSY15-H1(T)的九种糖脂和菌株 WSY15-H3(T)的七种糖脂；其中两种糖脂（GL1 和 GL3）在色谱上分别与双硫酸化二糖基醚（S2-DGD-1）和硫酸化二糖基醚（S-DGD-1）相同。菌株 WSY15-H1(T)和 WSY15-H3(T)的基因组 DNA G+C 含量分别为 65.4 和 66.2 mol%。基于 16S rRNA 基因序列分析，菌株 WSY15-H1(T)和 WSY15-H3(T)彼此之间的相似度为 97.0%，与唯一的模式种 Halolamina pelagica TBN21(T)的相似度分别为 98.4%和 97.6%，后者与这两株分离株的相似度高于 91%。系统发育关系和 DNA-DNA 同源性分析表明，菌株 WSY15-H1(T)和 WSY15-H3(T)代表两个与 H. pelagica TBN21(T)亲缘关系最密切的新谱系。基于表型、化学分类和基因型特征，提出了两个 Halolamina 属的新种，Halolamina salifodinae sp. nov.（模式菌株 WSY15-H1(T) = JCM 18548(T) = GCMCC 1.12371(T)）和 Halolamina salina sp. nov.（模式菌株 WSY15-H3(T) = JCM 18549(T) = GCMCC 1.12285(T)）。
