Sizova Maria, Panikov Nicolai
Stevens Institute of Technology, Arthur E. Imperatore School of Sciences and Arts, Castle Point on Hudson, Hoboken 07030, NJ, USA.
Int J Syst Evol Microbiol. 2007 Mar;57(Pt 3):616-619. doi: 10.1099/ijs.0.64350-0.
Psychrotolerant (0-25 degrees C), chemolithotrophic Gram-negative cocci were isolated from Alaskan forest soil. The novel isolate was found to grow autotrophically on H(2) : CO(2) mixtures and to switch to heterotrophic growth on media containing organic substrates. The novel strain utilized a wide range of organic acids, some simple sugars and alcohols. Naphthalene vapour did not support growth. On the basis of 16S rRNA gene sequence similarity, the novel strain is affiliated to the genus Polaromonas, of the class Betaproteobacteria, and is related to Polaromonas naphthalenivorans (99.6 % gene sequence similarity), Polaromonas aquatica (97.4 %) and Polaromonas vacuolata (96.1 %). The membrane phospholipids contained 16 : 1omega7c/16 : 1omega6c, 16 : 0 and 18 : 1omega7c, similar to the fatty acids found for P. naphthalenivorans, P. aquatica and P. vacuolata. On the basis of DNA-DNA hybridization, physiological and biochemical properties, the hydrogen-oxidizing mixotrophic isolate represents a novel species, for which the name Polaromonas hydrogenivorans sp. nov. is proposed. The type strain is DSM 17735(T) (=NRRL B-41369(T)).
从阿拉斯加森林土壤中分离出耐冷（0 - 25摄氏度）、化能无机营养型革兰氏阴性球菌。该新分离菌株能在H₂:CO₂混合气上自养生长，并在含有有机底物的培养基上转变为异养生长。该新菌株利用多种有机酸、一些单糖和醇类。萘蒸气不能支持其生长。基于16S rRNA基因序列相似性，该新菌株隶属于β-变形菌纲的极地单胞菌属，与萘食极地单胞菌（基因序列相似性99.6%）、水生极地单胞菌（97.4%）和液泡极地单胞菌（96.1%）相关。膜磷脂含有16:1ω7c/16:1ω6c、16:0和18:1ω7c，与萘食极地单胞菌、水生极地单胞菌和液泡极地单胞菌中发现的脂肪酸相似。基于DNA - DNA杂交、生理和生化特性，该氢氧化混合营养型分离菌株代表一个新物种，提议将其命名为食氢极地单胞菌（Polaromonas hydrogenivorans sp. nov.）。模式菌株为DSM 17735(T)（=NRRL B - 41369(T)）。
