HIV-1感染母亲母乳中HIV-1 RNA和DNA的多样性。

Diversity of HIV-1 RNA and DNA in breast milk from HIV-1-infected mothers.

作者信息

Becquart Pierre, Courgnaud Valerie, Willumsen Juana, Van de Perre Philippe

机构信息

Research Unit 145, UMR 145, Institute for Research and Development, University of Montpellier 1, Montpellier University Hospital, Montpellier, France.

出版信息

Virology. 2007 Jul 5;363(2):256-60. doi: 10.1016/j.virol.2007.02.003. Epub 2007 Mar 1.

DOI:10.1016/j.virol.2007.02.003

PMID:17335864

Abstract

We compared human immunodeficiency virus type 1 (HIV-1) RNA and DNA populations in the different fractions of breast milk (lactoserum, lipid layer, cell pellet) and between right and left breasts in four HIV-1-infected mothers by analyzing the hypervariable env C2-V5 region. Phylogenetic analyses of the viral quasispecies revealed that RNA populations and DNA populations were clearly distinct and that viral RNA sequences were similar in lipid layer and lactoserum in the milk of 3 out of 4 mothers. Comparison of viral DNA between milk from right and left breast showed a differential distribution of variants in three mothers. In contrast, RNA variants detected from milk of the two breasts were mixed in 3 out of 4 mothers. This study suggests that each mammary gland is subjected to microenvironmental pressure that may differ from the contralateral breast.

摘要

我们通过分析高变env C2-V5区域，比较了4名感染人类免疫缺陷病毒1型（HIV-1）的母亲母乳不同组分（乳清、脂质层、细胞沉淀）以及左右乳房之间的HIV-1 RNA和DNA群体。病毒准种的系统发育分析表明，RNA群体和DNA群体明显不同，并且在4名母亲中有3名母亲的乳汁中，脂质层和乳清中的病毒RNA序列相似。左右乳房乳汁中病毒DNA的比较显示，3名母亲中存在变异体的差异分布。相反，在4名母亲中有3名母亲的两侧乳房乳汁中检测到的RNA变异体是混合的。这项研究表明，每个乳腺都受到可能与对侧乳房不同的微环境压力。