Baldini A, Miller D A, Miller O J, Ryder O A, Mitchell A R
Department of Molecular Biology and Genetics, Wayne State University, Detroit, MI 48201.
Chromosoma. 1991 Mar;100(3):156-61. doi: 10.1007/BF00337244.
We describe a cloned 2.7 kb alpha satellite sequence, Pan-3, from the pygmy chimpanzee (Pan paniscus) that specifically hybridizes in situ to chromosome 19 in the pygmy chimpanzee and to the homeologous human chromosome, no. 17. Using high stringency conditions of hybridization on Southern blots, this sequence hybridized to DNA from both species of chimpanzee (P. paniscus and P. troglodytes) and from human but not to DNA from gorilla (Gorilla gorilla) or orangutan (Pongo pygmaeus). Partial sequence analysis showed that Pan-3 and a previously described human chromosome 17-specific clone have up to 91% sequence identity. To our knowledge this is the highest sequence similarity reported between alphoid subsets from human and any other primate.
我们描述了一种从倭黑猩猩（Pan paniscus）克隆得到的2.7 kbα卫星序列Pan-3，它在原位杂交中能特异性地与倭黑猩猩的19号染色体以及与之同源的人类17号染色体杂交。在Southern杂交中使用高严谨度杂交条件时，该序列能与两种黑猩猩（倭黑猩猩和普通黑猩猩）以及人类的DNA杂交，但不能与大猩猩（Gorilla gorilla）或猩猩（Pongo pygmaeus）的DNA杂交。部分序列分析表明，Pan-3与之前描述的人类17号染色体特异性克隆具有高达91%的序列同一性。据我们所知，这是报道的人类与任何其他灵长类动物α卫星亚群之间最高的序列相似性。
