一种在所有人类和大型猿类染色体中均保守的α卫星DNA序列：人类染色体区域2q21和9q13存在古老着丝粒序列的证据。
An alphoid DNA sequence conserved in all human and great ape chromosomes: evidence for ancient centromeric sequences at human chromosomal regions 2q21 and 9q13.
作者信息
Baldini A, Ried T, Shridhar V, Ogura K, D'Aiuto L, Rocchi M, Ward D C
机构信息
Department of Human Genetics, Yale University School of Medicine, New Haven, CT 06510.
出版信息
Hum Genet. 1993 Feb;90(6):577-83. doi: 10.1007/BF00202474.
Using vector-CENP-B box polymerase chain reaction (PCR) we isolated and cloned from a human chromosome 21-specific plasmid library, a 1 kb DNA sequence, named p alpha H21. In in situ hybridization experiments, p alpha H21 hybridized, under high stringency conditions, to the centromeric region of all the human, chimpanzee, gorilla and orangutan chromosomes. On human chromosomes p alpha H21 also identified non-centromeric sequences at 2q21 (locus D2F33S1) and 9q13 (locus D9F33S2). The possible derivation of these sequences from ancestral centromeres is discussed. Sequence analysis confirmed the alphoid nature of the whole p alpha H21 insert.
利用载体着丝粒蛋白B盒聚合酶链反应（PCR），我们从一个人类21号染色体特异性质粒文库中分离并克隆出一段1 kb的DNA序列，命名为pαH21。在原位杂交实验中，pαH21在高严格条件下与所有人类、黑猩猩、大猩猩和猩猩染色体的着丝粒区域杂交。在人类染色体上，pαH21还在2q21（基因座D2F33S1）和9q13（基因座D9F33S2）处鉴定出非着丝粒序列。本文讨论了这些序列可能源自祖先着丝粒的情况。序列分析证实了整个pαH21插入片段的α卫星性质。
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