Yerle M, Galman O, Lahbib-Mansais Y, Gellin J
INRA, Centre de Recherches de Toulouse, Laboratoire de Génétique Cellulaire, Castanet-Tolosan, France.
Cytogenet Cell Genet. 1992;59(1):48-51. doi: 10.1159/000133198.
The porcine gene for luteinizing hormone/choriogonadotropin receptor (LHCGR) was localized to chromosome 3q2.2----q2.3 using radioactive and nonradioactive in situ hybridization. A computer-assisted image-analysis system was developed which facilitated detection of the position of silver grains and fluorescent spots on the chromosomes after in situ hybridization. Compared with autoradiographic visualization, the nonisotopic procedure proved to be more rapid, precise, and highly specific; however, nonradiographic in situ hybridization was much less efficient than the autoradiographic technique for the detection of unique DNA sequences with small probes. From these results and published gene-mapping data, it was concluded that the synteny between LHCGR and MDH1 observed in man is conserved in the pig genome.
利用放射性和非放射性原位杂交技术，将猪促黄体激素/绒毛膜促性腺激素受体（LHCGR）基因定位到3号染色体的q2.2----q2.3区域。开发了一种计算机辅助图像分析系统，该系统有助于在原位杂交后检测染色体上银颗粒和荧光斑点的位置。与放射自显影可视化相比，非同位素方法被证明更快、更精确且特异性更高；然而，对于用小探针检测独特的DNA序列，非放射性原位杂交的效率远低于放射自显影技术。根据这些结果和已发表的基因定位数据，得出结论：在人类中观察到的LHCGR和MDH1之间的同线性在猪基因组中是保守的。
