Pitel F, Fillon V, Heimel C, Le Fur N, el Khadir-Mounier C, Douaire M, Gellin J, Vignal A
Laboratoire de Génétique Cellulaire, INRA-CRT, Castanet-Tolosan, France.
Mamm Genome. 1998 Apr;9(4):297-300. doi: 10.1007/s003359900752.
Fatty acid synthase and Acetyl-CoA carboxylase are both key enzymes of lipogenesis and may play a crucial role in the weight variability of abdominal adipose tissue in the growing chicken. They are encoded by the FASN and ACACA genes, located on human Chromosome (Chr) 17q25 and on Chr 17q12 or 17q21 respectively, a large region of conserved synteny among mammals. We have localized the homologous chicken genes FASN and ACACA coding for these enzymes, by single-strand conformation polymorphism analysis on different linkage groups of the Compton and East Lansing consensus genetic maps and by FISH on two different chicken microchromosomes. Although synteny is not conserved between these two genes, our results revealed linkage in chicken between FASN and NDPK (nucleoside diphosphate kinase), a homolog to the human NME1 and NME2 genes (non-metastatic cell proteins 1 and 2), both located on human Chr 17q21.3, and also between FASN and H3F3B (H3 histone family 3B), located on human Chr 17q25. The analysis of mapping data from the literature for other chicken and mammalian genes indicates rearrangements have occurred in this region in the mammalian lineage since the mammalian and avian radiation.
脂肪酸合酶和乙酰辅酶A羧化酶都是脂肪生成的关键酶，可能在生长中的鸡腹部脂肪组织的体重变异性中起关键作用。它们分别由位于人类染色体（Chr）17q25上的FASN基因和位于Chr 17q12或17q21上的ACACA基因编码，这是哺乳动物中一个保守的大片段同线性区域。我们通过对康普顿和东兰辛共识遗传图谱的不同连锁群进行单链构象多态性分析，并通过荧光原位杂交技术在两条不同的鸡微小染色体上，定位了编码这些酶的同源鸡基因FASN和ACACA。尽管这两个基因之间的同线性不保守，但我们的结果显示，鸡的FASN与NDPK（核苷二磷酸激酶，与人类NME1和NME2基因（非转移性细胞蛋白1和2）同源，均位于人类Chr 17q21.3）之间存在连锁，同时FASN与位于人类Chr 17q25上的H3F3B（H3组蛋白家族3B）之间也存在连锁。对来自文献的其他鸡和哺乳动物基因的定位数据的分析表明，自哺乳动物和鸟类分化以来，该区域在哺乳动物谱系中发生了重排。
