Fielding C Langdon, Magdesian K Gary, Carlson Gary P, Ruby Rebecca E, Rhodes Diane M
Loomis Basin Large Animal Veterinary Hospital, CA, USA.
J Vet Intern Med. 2007 Jan-Feb;21(1):176-83. doi: 10.1892/0891-6640(2007)21[176:eoafsu]2.0.co;2.
Multi-frequency bioelectrical impedance analysis (MF-BIA) has been used to evaluate extracellular fluid volume (ECFV), but not fluid fluxes associated with fluid or furosemide administration in horses. If able to detect acute changes in ECFV, MF-BIA would be useful in monitoring fluid therapy in horses.
The purpose of this study was to evaluate the ability of MF-BIA to detect acute fluid compartment changes in horses. We hypothesized that MF-BIA would detect clinically relevant (10-20%) changes in ECFV.
Six healthy mares were used in the study.
This is an original experimental study. Mares were studied in 3 experiments: (1) crystalloid expansion of normally hydrated subjects, (2) furosemide-induced dehydration followed by crystalloid administration, and (3) acute blood loss followed by readministration of lost blood. MF-BIA measurements were made before, during, and after each fluid shift and compared to known changes in volume calculated based on the intravenous fluids that were administered in addition to urinary fluid losses. Mean errors between MF-BIA estimated change and known volume change were compared using nonparametric analysis of variance. Estimated ECFV pre- and post-fluid administration similarly were compared. The level of statistical significance was set at P < .05.
Results of the study revealed a statistically significant change in ECFV and total body water during crystalloid expansion and dehydration. Statistically significant changes were not observed during blood loss and administration. Mean errors between MF-BIA results and measured net changes were small.
MF-BIA represents a practical and accurate means of assessing acute fluid changes during dehydration and expansion of ECFV using isotonic crystalloids with potential clinical applications in equine critical care.
多频生物电阻抗分析（MF-BIA）已被用于评估细胞外液量（ECFV），但尚未用于评估马匹中与补液或呋塞米给药相关的液体通量。如果能够检测到ECFV的急性变化，MF-BIA将有助于监测马匹的液体疗法。
本研究的目的是评估MF-BIA检测马匹急性液体腔室变化的能力。我们假设MF-BIA将检测到ECFV临床上相关的（10%-20%）变化。
本研究使用了6匹健康母马。
这是一项原创性实验研究。母马在3个实验中接受研究：（1）正常水合状态的受试者进行晶体液扩容；（2）呋塞米诱导脱水后进行晶体液给药；（3）急性失血后再输注丢失的血液。在每次液体转移之前、期间和之后进行MF-BIA测量，并与基于除尿液液体损失外所输注的静脉液体计算出的已知体积变化进行比较。使用非参数方差分析比较MF-BIA估计变化与已知体积变化之间的平均误差。同样比较液体给药前后估计的ECFV。统计学显著性水平设定为P < 0.05。
研究结果显示，在晶体液扩容和脱水期间，ECFV和总体水有统计学显著变化。在失血和输血期间未观察到统计学显著变化。MF-BIA结果与测量的净变化之间的平均误差较小。
MF-BIA是一种实用且准确的方法，可用于评估使用等渗晶体液进行脱水和ECFV扩容期间的急性液体变化，在马的重症监护中具有潜在的临床应用价值。
