Forro M, Cieslar S, Ecker G L, Walzak A, Hahn J, Lindinger M I
Department of Human Biology and Nutritional Sciences, University of Guelph, Ontario, Canada.
J Appl Physiol (1985). 2000 Aug;89(2):663-71. doi: 10.1152/jappl.2000.89.2.663.
The purposes of this study were 1) to determine the compartmentation of body water in horses by using indicator dilution techniques and 2) to simultaneously measure bioelectrical impedance to current flow at impulse current frequencies of 5 and 200 kHz to formulate predictive equations that could be used to estimate total body water (TBW), extracellular fluid volume (ECFV), and intracellular fluid volume (ICFV). Eight horses and ponies weighing from 214 to 636 kg had catheters placed into the left and right jugular veins. Deuterium oxide, sodium thiocyanate, and Evans blue were infused for the measurement of TBW, ECFV, and plasma volume (PV), respectively. Bioelectrical impedance was measured by using a tetrapolar electrode configuration, with electrode pairs secured above the knee and hock. Measured TBW, ECFV, and PV were 0.677 +/- 0.022, 0.253 +/- 0.006, and 0.040 +/- 0.002 l/kg body mass, respectively. Strong linear correlations were determined among measured variables that allowed for the prediction of TBW, ECFV, ICFV, and PV from measures of horse length or height and impedance. It is concluded that bioelectrical impedance analysis (BIA) can be used to improve the predictive accuracy of noninvasive estimates of ECFV and PV in euhydrated horses at rest.
1）通过使用指示剂稀释技术确定马体内水的分布情况；2）同时测量5 kHz和200 kHz脉冲电流频率下电流流动的生物电阻抗，以制定可用于估计总体水（TBW）、细胞外液量（ECFV）和细胞内液量（ICFV）的预测方程。八匹体重在214至636 kg之间的马和矮种马，其左、右颈静脉均放置了导管。分别注入重水、硫氰酸钠和伊文思蓝以测量TBW、ECFV和血浆量（PV）。使用四极电极配置测量生物电阻抗，电极对固定在膝盖和跗关节上方。测得的TBW、ECFV和PV分别为0.677±0.022、0.253±0.006和0.040±0.002 l/kg体重。在测量变量之间确定了强线性相关性，这使得可以根据马的体长或身高以及阻抗测量值来预测TBW、ECFV、ICFV和PV。得出的结论是，生物电阻抗分析（BIA）可用于提高对处于静息状态的正常水合马匹的ECFV和PV进行无创估计的预测准确性。
