六种不同培养基用于猪痢疾密螺旋体分离的比较
Comparison of six different culture media for isolation of Treponema hyodysenteriae.
作者信息
Achacha M, Messier S
机构信息
Faculté de Médecine Vétérinaire, Université de Montréal, St-Hyacinthe, Québec, Canada.
出版信息
J Clin Microbiol. 1992 Jan;30(1):249-51. doi: 10.1128/jcm.30.1.249-251.1992.
Fecal material from pigs experimentally infected with Treponema hyodysenteriae was collected and inoculated on six different selective media to compare their abilities for recovery of T. hyodysenteriae. Additionally, various types of samples were compared to find the most appropriate for submitting material to laboratories. Isolation rates for T. hyodysenteriae were 89.7% on BJ medium, 88.3% on TSA-BJ medium, 76.6% on SVC medium, 76.6% on BA2-BJ medium, 75.2% on BA2-SVC medium, and 52.4% on TSA-S400 medium. BJ medium was the most useful, yielding good growth of T. hyodysenteriae and inhibition of the normal fecal flora. Also, undiluted fecal material kept at refrigeration temperature allowed better recovery of T. hyodysenteriae during storage than swabs.
收集来自实验感染猪痢疾短螺旋体的猪的粪便样本，并接种于六种不同的选择性培养基上，以比较它们分离猪痢疾短螺旋体的能力。此外，还比较了各种类型的样本，以找出最适合提交给实验室的样本类型。猪痢疾短螺旋体在BJ培养基上的分离率为89.7%，在TSA-BJ培养基上为88.3%，在SVC培养基上为76.6%，在BA2-BJ培养基上为76.6%，在BA2-SVC培养基上为75.2%，在TSA-S400培养基上为52.4%。BJ培养基最有用，猪痢疾短螺旋体生长良好，且能抑制正常粪便菌群。此外，与拭子相比，冷藏温度下保存的未稀释粪便样本在储存期间能更好地分离出猪痢疾短螺旋体。
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