Burrows M R, Lemcke R M
Vet Rec. 1981 Feb 28;108(9):187-9. doi: 10.1136/vr.108.9.187.
A rapid slide agglutination (SA) test was developed to identify the spirochaete Treponema hyodysenteriae, the causative organism of swine dysentery. The specificity of the antiserum was increased by a single absorption with two intestinal spirochaetes. Using this test, it was possible to identify 30 out of 31 spirochaetes which were beta-haemolytic and gave a positive reaction in growth inhibition (GI) tests with T hyodysenteriae antiserum. All except one of these spirochaetes were isolated from herds with a history of swine dysentery or suspected swine dysentery. The majority of the spirochaetes gave a rapid, strongly, positive reaction in the SA test but seven strains, although recognisably positive, reacted more weakly. Of 28 other spirochaetes which were weakly beta-haemolytic and did not react in GI tests with T hyodysenteriae antiserum, 27 were negative in the SA test. The remaining strain was autoagglutinable and thus could not be identified. The indole test correlated less well with the results of SA and GI tests. All 31 strains which were identified as T hyodysenteriae produced indole, but so did nine of the 28 other spirochaetes. The slide agglutination test is a potentially useful method for rapid identification of T hyodysenteriae.
开发了一种快速玻片凝集（SA）试验，以鉴定猪痢疾的病原体——螺旋体猪痢疾密螺旋体。通过用两种肠道螺旋体进行一次吸收，提高了抗血清的特异性。使用该试验，可以鉴定出31株螺旋体中的30株，这些螺旋体具有β-溶血特性，并且在用猪痢疾密螺旋体抗血清进行的生长抑制（GI）试验中呈阳性反应。除一株外，所有这些螺旋体均从有猪痢疾病史或疑似猪痢疾的猪群中分离得到。大多数螺旋体在SA试验中呈快速、强烈的阳性反应，但有7株虽然明显呈阳性，但反应较弱。在其他28株弱β-溶血且在用猪痢疾密螺旋体抗血清进行的GI试验中无反应的螺旋体中，27株在SA试验中呈阴性。其余菌株可自身凝集，因此无法鉴定。吲哚试验与SA和GI试验结果的相关性较差。所有31株被鉴定为猪痢疾密螺旋体的菌株都产生吲哚，但其他28株螺旋体中的9株也产生吲哚。玻片凝集试验是一种快速鉴定猪痢疾密螺旋体的潜在有用方法。
