严重急性呼吸综合征冠状病毒辅助蛋白6是一种病毒体相关蛋白，可从表达6蛋白的细胞中释放出来。
Severe acute respiratory syndrome coronavirus accessory protein 6 is a virion-associated protein and is released from 6 protein-expressing cells.
作者信息
Huang Cheng, Peters C J, Makino Shinji
机构信息
Department of Microbiology and Immunology, The University of Texas Medical Branch at Galveston, Galveston, TX 77555-1019, USA.
出版信息
J Virol. 2007 May;81(10):5423-6. doi: 10.1128/JVI.02307-06. Epub 2007 Mar 7.
Abstract
Analysis of severe acute respiratory syndrome coronavirus (SCoV) by either sucrose gradient equilibrium centrifugation or a virus capture assay using an anti-SCoV S protein antibody demonstrated that the SCoV 6 protein, which is one of the accessory proteins of SCoV, was incorporated into virus particles. Coexpression of the SCoV S, M, E, and 6 proteins was sufficient for incorporation of the 6 protein into virus-like particles. Cells transfected with plasmid expressing the 6 protein released SCoV 6 protein; however, infected cells released SCoV 6 protein only in association with SCoV particles.
摘要
通过蔗糖梯度平衡离心或使用抗SCoV S蛋白抗体的病毒捕获试验对严重急性呼吸综合征冠状病毒（SCoV）进行分析，结果表明，SCoV的辅助蛋白之一SCoV 6蛋白被整合到病毒颗粒中。SCoV的S、M、E和6蛋白的共表达足以使6蛋白整合到病毒样颗粒中。用表达6蛋白的质粒转染的细胞释放出SCoV 6蛋白；然而，被感染的细胞仅在与SCoV颗粒相关联时才释放SCoV 6蛋白。
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