Donova M V
Prikl Biokhim Mikrobiol. 2007 Jan-Feb;43(1):5-18.
Development of pharmaceutical industry is currently aimed at introducing biotechnological processes on a large-scale and thereby replacing multiple-stage chemical syntheses. Actinobacteria are efficient biocatalysts of many processes involving steroid bioconversion, which hold considerable importance for the synthesis of hormonal drugs. The potential to catalyze the conversion of a broad spectrum of steroid substrates makes it possible to expect efficient utilization of these microorganisms in development of new technologies of manufacturing steroid pharmaceutical substances. The review is a first attempt to systematize data on the potential of actinobacteria to catalyze diverse reactions of steroid transformation (such as hydroxylation, introduction and reduction of double bonds, oxidation of steroid alcohols, reduction of ketones, side chain de-esterification and degradation, etc.), with emphasis on processes of practical biotechnological importance and progress in steroid bioconversion over the last ten years.
制药行业的发展目前旨在大规模引入生物技术工艺，从而取代多步化学合成。放线菌是许多涉及甾体生物转化过程的高效生物催化剂，这对激素药物的合成具有相当重要的意义。催化多种甾体底物转化的潜力使得有望在甾体药物物质制造新技术的开发中有效利用这些微生物。这篇综述首次尝试系统整理关于放线菌催化甾体转化各种反应（如羟基化、双键的引入和还原、甾体醇的氧化、酮的还原、侧链脱酯化和降解等）潜力的数据，重点关注具有实际生物技术重要性的过程以及过去十年甾体生物转化的进展。
