含磷脂心磷脂和氟喹诺酮左氧氟沙星的脂质体对广泛耐药结核分枝杆菌的抗菌作用

Antibacterial Effects of Liposomes Containing Phospholipid Cardiolipin and Fluoroquinolone Levofloxacin on Mycobacterium tuberculosis with Extensive Drug Resistance.

作者信息

Gaidukevich S K, Mikulovich Yu L, Smirnova T G, Andreevskaya S N, Sorokoumova G M, Chernousova L N, Selishcheva A A, Shvets V I

机构信息

M. V. Lomonosov Moscow State University of Fine Chemical Technologies, Moscow, Russia.

Central Research Institute of Tuberculosis, Russian Academy of Medical Sciences, Moscow, Russia.

出版信息

Bull Exp Biol Med. 2016 Mar;160(5):675-8. doi: 10.1007/s10517-016-3247-z. Epub 2016 Mar 29.

DOI:10.1007/s10517-016-3247-z

PMID:27021087

Abstract

The effects of liposomes containing phospholipid cardiolipin without antibiotic and loaded with levofloxacin on the growth of Mycobacterium tuberculosis with extensive drug resistance were studied in vitro. Liposomes consisting of cardiolipin alone in a concentration of 335 μM completely suppressed the growth of M. tuberculosis. In order to reduce the minimum inhibitory concentration of cardiolipin, complex liposome preparation consisting of phosphatidylcholin/cholesterol/cardiolipin and loaded with levofloxacin was prepared. Due to this, the cardiolipin concentration was reduced to 33.5 μM (50 μg/ml) and concentration of levofloxacin - to 2 μg/ml.

摘要

研究了不含抗生素但负载左氧氟沙星的含磷脂心磷脂脂质体对广泛耐药结核分枝杆菌生长的体外影响。浓度为335μM的仅由心磷脂组成的脂质体完全抑制了结核分枝杆菌的生长。为了降低心磷脂的最低抑菌浓度，制备了由磷脂酰胆碱/胆固醇/心磷脂组成并负载左氧氟沙星的复合脂质体制剂。由此，心磷脂浓度降至33.5μM（50μg/ml），左氧氟沙星浓度降至2μg/ml。