耻垢分枝杆菌的丙氨酸消旋酶在缺乏D-丙氨酸的情况下对生长至关重要。
The alanine racemase of Mycobacterium smegmatis is essential for growth in the absence of D-alanine.
作者信息
Milligan Daniel L, Tran Sieu L, Strych Ulrich, Cook Gregory M, Krause Kurt L
机构信息
Department of Biochemistry, Otago School of Medical Sciences, University of Otago, P.O. Box 56, Dunedin, New Zealand.
出版信息
J Bacteriol. 2007 Nov;189(22):8381-6. doi: 10.1128/JB.01201-07. Epub 2007 Sep 7.
Abstract
Alanine racemase, encoded by the gene alr, is an important enzyme in the synthesis of d-alanine for peptidoglycan biosynthesis. Strains of Mycobacterium smegmatis with a deletion mutation of the alr gene were found to require d-alanine for growth in both rich and minimal media. This indicates that alanine racemase is the only source of d-alanine for cell wall biosynthesis in M. smegmatis and confirms alanine racemase as a viable target gene for antimycobacterial drug development.
摘要
由alr基因编码的丙氨酸消旋酶是肽聚糖生物合成中d - 丙氨酸合成的重要酶。发现耻垢分枝杆菌中alr基因缺失突变的菌株在丰富培养基和基本培养基中生长都需要d - 丙氨酸。这表明丙氨酸消旋酶是耻垢分枝杆菌细胞壁生物合成中d - 丙氨酸的唯一来源，并证实丙氨酸消旋酶是抗分枝杆菌药物开发的一个可行靶基因。
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