Stock Sarah J, Kelly Rodney W, Riley Simon C, Calder Andrew A
Department of Obstetrics and Gynaecology, University of Edinburgh, Centre for Reproductive Biology, Queen's Medical Research Institute, Edinburgh, UK.
Am J Obstet Gynecol. 2007 Mar;196(3):255.e1-6. doi: 10.1016/j.ajog.2006.10.908.
The purpose of this study was to determine the expression of natural antimicrobials in primary cultured amnion epithelial cells and to examine their regulation by interleukin-1 beta (IL-1beta).
Primary amnion epithelial cells were cultured from samples that were obtained at prelabor cesarean section (n = 12) and stimulated with IL-1beta. Natural antimicrobial messenger RNA expression was determined by real-time quantitative polymerase chain reaction, and protein was measured by enzyme-linked immunosorbent assay. Data was analyzed by analysis of variance.
Primary amnion epithelial cells express messenger RNA for human beta defensin (HBD) 1 to 3, secretory leukocyte protease inhibitor and elafin, but not HBD4. IL-1beta 10 ng/mL stimulates HBD2 messenger RNA in a biphasic pattern, with a 51-fold increase at 6 hours and a 67-fold at 12 hours (P < .001). HBD2 protein production is significantly increased by 24 hours (P < .05).
The amnion produces potent natural antimicrobials that may help protect the pregnancy from infection. HBD2 production is dramatically upregulated by the labor-associated inflammatory cytokine IL-1beta.
本研究旨在确定原代培养的羊膜上皮细胞中天然抗菌物质的表达，并检测白细胞介素-1β（IL-1β）对其的调节作用。
从临产前剖宫产获取的样本中培养原代羊膜上皮细胞（n = 12），并用IL-1β进行刺激。通过实时定量聚合酶链反应测定天然抗菌信使核糖核酸的表达，用酶联免疫吸附测定法测量蛋白质。数据采用方差分析。
原代羊膜上皮细胞表达人β-防御素（HBD）1至3、分泌型白细胞蛋白酶抑制剂和弹性蛋白酶的信使核糖核酸，但不表达HBD4。10 ng/mL的IL-1β以双相模式刺激HBD2信使核糖核酸，6小时时增加51倍，12小时时增加67倍（P <.001）。HBD2蛋白产量在24小时时显著增加（P <.05）。
羊膜产生强效天然抗菌物质，可能有助于保护孕期免受感染。与分娩相关的炎性细胞因子IL-1β可显著上调HBD2的产生。
